Focal adhesion disassembly requires clathrin‐dependent endocytosis of integrins

FEBS Letters - Tập 583 - Trang 1337-1343 - 2009
Wei-Ting Chao1, Jeannette Kunz1
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 1 Baylor Plaza, BCM335, RM T419, Houston, TX 77030, USA

Tóm tắt

Cell migration requires the controlled disassembly of focal adhesions, but the underlying mechanisms remain poorly understood. Here, we show that adhesion turnover is mediated through dynamin‐ and clathrin‐dependent endocytosis of activated β1 integrins. Consistent with this, clathrin and the clathrin adaptors AP‐2 and disabled‐2 (DAB2) distribute along with dynamin 2 to adhesion sites prior to adhesion disassembly. Moreover, knockdown of either dynamin 2 or both clathrin adaptors blocks β1 integrin internalization, leading to impaired focal adhesion disassembly and cell migration. Together, these results provide important insight into the mechanisms underlying adhesion disassembly and identify novel components of the disassembly pathway.

Tài liệu tham khảo

10.1038/ncb0402-e97 10.1016/j.ceb.2007.10.009 10.1038/ncb1094 10.1038/ncb1175 10.1038/ncb1262 10.1038/nature01451 10.1073/pnas.0510306103 10.1016/S1097-2765(00)80398-6 10.1242/jcs.03383 10.1016/j.devcel.2007.05.003 10.1091/mbc.E03-01-0046 10.1007/978-1-59745-178-9_2 10.1152/ajpcell.00160.2006 10.1093/emboj/cdf487 10.1083/jcb.200309175 10.1083/jcb.150.5.1137 10.1016/j.tcb.2008.03.004 10.1042/BJ20081288 10.1126/science.7569928 10.1146/annurev.biochem.72.121801.161800 10.1034/j.1600-0854.2001.020206.x 10.1002/j.1460-2075.1996.tb00965.x 10.1083/jcb.117.6.1321 10.1083/jcb.141.2.527 10.1006/bbrc.1994.1271 10.1091/mbc.E06-05-0421
Thông tin
Thông tin xuất bản

FEBS Letters

Tập 583

1337-1343

Thông tin tác giả