Mechanism for Regulating Cell Surface Distribution of Na <sup>+</sup> ,K <sup>+</sup> -ATPase in Polarized Epithelial Cells

American Association for the Advancement of Science (AAAS) - Tập 254 Số 5033 - Trang 847-850 - 1991
R W Hammerton1, Kathleen A. Krzeminski1, Robert Mays1, Timothy A. Ryan1, Debra A. Wollner1, W. James Nelson1
1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305-5426.

Tóm tắt

Restriction of sodium, potassium adenosine triphosphatase (Na + ,K + -ATPase) to either the apical or basal-lateral membrane domain of polarized epithelial cells is fundamental to vectorial ion and solute transport in many tissues and organs. A restricted membrane distribution of Na + ,K + -ATPase in Madin-Darby canine kidney (MDCK) epithelial cells was found experimentally to be generated by preferential retention of active enzyme in the basal-lateral membrane domain and selective inactivation and loss from the apical membrane domain, rather than by vectorial targeting of newly synthesized protein from the Golgi complex to the basal-lateral membrane domain. These results show how different distributions of the same subunits of Na + ,K + -ATPase may be generated in normal polarized epithelia and in disease states.

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Tài liệu tham khảo

AVNER E unpublished data.

BACALLAO, R, THE SUBCELLULAR ORGANIZATION OF MADIN-DARBY CANINE KIDNEY-CELLS DURING THE FORMATION OF A POLARIZED EPITHELIUM, JOURNAL OF CELL BIOLOGY 109: 2817 (1989).

Caplan, M., Modern Cell Biology 8: 71 (1989).

CAPLAN, M.J., INTRACELLULAR SORTING AND POLARIZED CELL-SURFACE DELIVERY OF (NA+,K+)ATPASE, AN ENDOGENOUS COMPONENT OF MDCK CELL BASOLATERAL PLASMA-MEMBRANES, CELL 46: 623 (1986).

COHEN, S.M., IMMUNOCHEMICAL CHARACTERIZATION OF RELATED FAMILIES OF GLYCOPROTEINS IN DESMOSOMES, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 2621 (1983).

CONTRERAS, R.G., REPOLARIZATION OF NA+-K+ PUMPS DURING ESTABLISHMENT OF EPITHELIAL MONOLAYERS, AMERICAN JOURNAL OF PHYSIOLOGY 257: C896 (1989).

EKBLOM, P, CELL-MATRIX INTERACTIONS AND CELL-ADHESION DURING DEVELOPMENT, ANNUAL REVIEW OF CELL BIOLOGY 2: 27 (1986).

GHOSH, S, CELLULAR-DISTRIBUTION AND DIFFERENTIAL GENE-EXPRESSION OF THE 3 ALPHA-SUBUNIT ISOFORMS OF THE NA,K-ATPASE IN THE OCULAR CILIARY EPITHELIUM, JOURNAL OF BIOLOGICAL CHEMISTRY 265: 2935 (1990).

GUNDERSEN, D, APICAL POLARITY OF NA,K-ATPASE IN RETINAL-PIGMENT EPITHELIUM IS LINKED TO A REVERSAL OF THE ANKYRIN-FODRIN SUBMEMBRANE CYTOSKELETON, JOURNAL OF CELL BIOLOGY 112: 863 (1991).

HAMMERTON R.W. unpublished data.

HUBBARD, A.L., BIOGENESIS OF ENDOGENOUS PLASMA-MEMBRANE PROTEINS IN EPITHELIAL-CELLS, ANNUAL REVIEW OF PHYSIOLOGY 51: 755 (1989).

JORGENSEN, P.L., MECHANISM OF THE NA+, K+ PUMP PROTEIN-STRUCTURE AND CONFORMATIONS OF THE PURE (NA+ +K+)-ATPASE, BIOCHIMICA ET BIOPHYSICA ACTA 694: 27 (1982).

KASHGARIAN, M, MONOCLONAL-ANTIBODY TO NA,K-ATPASE - IMMUNOCYTOCHEMICAL LOCALIZATION ALONG NEPHRON SEGMENTS, KIDNEY INTERNATIONAL 28: 899 (1985).

KELLY, R.A., IDENTIFICATION OF NAK-ATPASE INHIBITORS IN HUMAN-PLASMA AS NONESTERIFIED FATTY-ACIDS AND LYSOPHOSPHOLIPIDS, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 11704 (1986).

KOOB, R, EUR J CELL BIOL 45: 230 (1987).

MARRS, J.A., JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 1: 711 (1990).

MATTER, K, SORTING OF ENDOGENOUS PLASMA-MEMBRANE PROTEINS OCCURS FROM 2 SITES IN CULTURED HUMAN INTESTINAL EPITHELIAL-CELLS (CACO-2), CELL 60: 429 (1990).

MCNEILL, H, NOVEL FUNCTION OF THE CELL-ADHESION MOLECULE UVOMORULIN AS AN INDUCER OF CELL-SURFACE POLARITY, CELL 62: 309 (1990).

MOLITORIS, B.A., CHARACTERIZATION OF ISCHEMIA-INDUCED LOSS OF EPITHELIAL POLARITY, JOURNAL OF MEMBRANE BIOLOGY 106: 233 (1988).

MORROW, J.S., ANKYRIN LINKS FODRIN TO THE ALPHA-SUBUNIT OF NA,K-ATPASE IN MADIN-DARBY CANINE KIDNEY-CELLS AND IN INTACT RENAL TUBULE CELLS, JOURNAL OF CELL BIOLOGY 108: 455 (1989).

NELSON, W.J., MODULATION OF FODRIN (MEMBRANE SKELETON) STABILITY BY CELL CELL CONTACT IN MADIN DARBY CANINE KIDNEY EPITHELIAL-CELLS, JOURNAL OF CELL BIOLOGY 104: 1527 (1987).

NELSON, W.J., A MEMBRANE CYTOSKELETAL COMPLEX CONTAINING NA+,K+-ATPASE, ANKYRIN, AND FODRIN IN MADIN DARBY CANINE KIDNEY (MDCK) CELLS - IMPLICATIONS FOR THE BIOGENESIS OF EPITHELIAL-CELL POLARITY, JOURNAL OF CELL BIOLOGY 108: 893 (1989).

NELSON, W.J., IDENTIFICATION OF A MEMBRANE CYTOSKELETAL COMPLEX CONTAINING THE CELL-ADHESION MOLECULE UVOMORULIN (E-CADHERIN), ANKYRIN, AND FODRIN IN MADIN-DARBY CANINE KIDNEY EPITHELIAL-CELLS, JOURNAL OF CELL BIOLOGY 110: 349 (1990).

NELSON, W.J., ANKYRIN BINDING TO (NA+ + K+)ATPASE AND IMPLICATIONS FOR THE ORGANIZATION OF MEMBRANE DOMAINS IN POLARIZED CELLS, NATURE 328: 533 (1987).

OJAKIAN, G.K., THE POLARIZED DISTRIBUTION OF AN APICAL CELL-SURFACE GLYCOPROTEIN IS MAINTAINED BY INTERACTIONS WITH THE CYTOSKELETON OF MADIN-DARBY CANINE KIDNEY-CELLS, JOURNAL OF CELL BIOLOGY 107: 2377 (1988).

PASDAR, M, REGULATION OF DESMOSOME ASSEMBLY IN EPITHELIAL-CELLS - KINETICS OF SYNTHESIS, TRANSPORT, AND STABILIZATION OF DESMOGLEIN-I, A MAJOR PROTEIN OF THE MEMBRANE CORE DOMAIN, JOURNAL OF CELL BIOLOGY 109: 163 (1989).

RODRIGUEZBOULAN, E, MORPHOGENESIS OF THE POLARIZED EPITHELIAL-CELL PHENOTYPE, SCIENCE 245: 718 (1989).

SARGIACOMO, M, INTEGRAL AND PERIPHERAL PROTEIN-COMPOSITION OF THE APICAL AND BASOLATERAL MEMBRANE DOMAINS IN MDCK CELLS, JOURNAL OF MEMBRANE BIOLOGY 107: 277 (1989).

SIMONS, K, CELL-SURFACE POLARITY IN EPITHELIA, ANNUAL REVIEW OF CELL BIOLOGY 1: 243 (1985).

SIMONS, K, LIPID SORTING IN EPITHELIAL-CELLS, BIOCHEMISTRY 27: 6197 (1988).

STAHL, W.L., THE NA,K-ATPASE OF NERVOUS-TISSUE, NEUROCHEMISTRY INTERNATIONAL 8: 449 (1986).

SUTHERLAND, E, BIOCHEMICAL LOCALIZATION OF HEPATIC SURFACE-MEMBRANE NA+,K+-ATPASE ACTIVITY DEPENDS ON MEMBRANE LIPID FLUIDITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 8673 (1988).

SWEADNER, K.J., ISOZYMES OF THE NA+/K+-ATPASE, BIOCHIMICA ET BIOPHYSICA ACTA 988: 185 (1989).

10.1073/pnas.76.9.4350

VONBONSDORFF, C.H., APICAL AND BASOLATERAL ENDOCYTOSIS IN MADIN-DARBY CANINE KIDNEY (MDCK) CELLS GROWN ON NITROCELLULOSE FILTERS, EMBO JOURNAL 4: 2781 (1985).

WILSON, P.D., REVERSED POLARITY OF NA+-K+-ATPASE - MISLOCATION TO APICAL PLASMA-MEMBRANES IN POLYCYSTIC KIDNEY-DISEASE EPITHELIA, AMERICAN JOURNAL OF PHYSIOLOGY 260: F420 (1991).

WOLLNER D unpublished data.

WRIGHT, E.M., MECHANISMS OF ION TRANSPORT ACROSS CHOROID-PLEXUS, JOURNAL OF PHYSIOLOGY-LONDON 226: 545 (1972).

ZHENG, B, INHIBITION OF PROTEIN KINASE-C, (SODIUM PLUS POTASSIUM)-ACTIVATED ADENOSINE-TRIPHOSPHATASE, AND SODIUM-PUMP BY SYNTHETIC PHOSPHOLIPID ANALOGS, CANCER RESEARCH 50: 3025 (1990).

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American Association for the Advancement of Science (AAAS)

Tập 254 Số 5033

847-850

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