Effect and possible mechanisms of melatonin treatment on the quality and developmental potential of aged bovine oocytes

Reproduction, Fertility and Development - Tập 29 Số 9 - Trang 1821 - 2017
Shuang Liang1, Jing Guo1, Jeong‐Woo Choi1, Nam‐Hyung Kim1, Xiang‐Shun Cui1
1Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea

Tóm tắt

After reaching the metaphase II (MII) stage, unfertilised oocytes undergo a time-dependent process of quality deterioration referred to as oocyte aging. The associated morphological and cellular changes lead to decreased oocyte developmental potential. This study investigated the effect of exogenous melatonin supplementation on in vitro aged bovine oocytes and explored its underlying mechanisms. The levels of cytoplasmic reactive oxygen species and DNA damage response in bovine oocytes increased during in vitro aging. Meanwhile, maturation promoting factor activity significantly decreased and the proportion of morphologically abnormal oocytes significantly increased. Melatonin supplementation significantly decreased quality deterioration in aged bovine MII oocytes (P < 0.05). Additionally, it decreased the frequency of aberrant spindle organisation and cortical granule release during oocyte aging (P < 0.05). In the melatonin-supplemented group, mitochondrial membrane potential and ATP production were significantly increased compared with control. Furthermore, melatonin treatment significantly increased the speed of development of bovine oocytes to the blastocyst stage after in vitro fertilisation and significantly decreased the apoptotic rate in the blastocysts (P < 0.05). The expression of Bax and Casp3 in the blastocysts was significantly reduced after treatment with melatonin, whereas expression of Bcl2 significantly increased (P < 0.05). In conclusion, these findings suggest that supplementation of aged bovine oocytes with exogenous melatonin improves oocyte quality, thereby enhancing the developmental capacity of early embryos.

Từ khóa


Tài liệu tham khảo

Abecia, 2002, Vet. Res. Commun., 26, 151, 10.1023/A:1014099719034

Agung, 2006, J. Reprod. Dev., 52, 123, 10.1262/jrd.17055

Alikani, 2000, Hum. Reprod., 15, 2634, 10.1093/humrep/15.12.2634

Arlotto, 1996, Theriogenology, 45, 943, 10.1016/0093-691X(96)00024-6

Asano, 2012, J. Histochem. Cytochem., 60, 229, 10.1369/0022155411431734

Bai, 2006, Mol. Reprod. Dev., 73, 1448, 10.1002/mrd.20538

Ben-Meir, 2015, Aging Cell, 14, 887, 10.1111/acel.12368

Bogliolo, 2013, J. Assist. Reprod. Genet., 30, 877, 10.1007/s10815-013-0046-6

Brzezinski, 1987, J. Clin. Endocrinol. Metab., 64, 865, 10.1210/jcem-64-4-865

Chi, 1988, Biol. Reprod., 39, 295, 10.1095/biolreprod39.2.295

Choi, 2007, Fertil. Steril., 88, 1220, 10.1016/j.fertnstert.2007.02.067

Choi, 2008, Mol. Reprod. Dev., 75, 1127, 10.1002/mrd.20861

Djahanbakhch, 2007, J. Pathol., 211, 219, 10.1002/path.2108

El-Raey, 2014, J. Reprod. Infertil., 5, 1

Fadini, 2012, Hum. Reprod., 27, 3601, 10.1093/humrep/des359

Fissore, 2002, Reproduction, 124, 745, 10.1530/rep.0.1240745

Fu, 2011, J. Pineal Res., 51, 104, 10.1111/j.1600-079X.2011.00867.x

Gao, 2012, J. Pineal Res., 52, 305, 10.1111/j.1600-079X.2011.00944.x

Garc�a, 2014, J. Pineal Res., 56, 225, 10.1111/jpi.12128

Heggeness, 1978, Proc. Natl. Acad. Sci. USA, 75, 3863, 10.1073/pnas.75.8.3863

Hooper, 2015, J. Reprod. Dev., 61, 459, 10.1262/jrd.2014-168

Huo, 2004, Biol. Reprod., 71, 853, 10.1095/biolreprod.104.028134

Ishizuka, 2000, J. Pineal Res., 28, 48, 10.1034/j.1600-079x.2000.280107.x

Juetten, 1983, Gamete Res., 8, 219, 10.1002/mrd.1120080303

Kang, 2009, J. Pineal Res., 46, 22, 10.1111/j.1600-079X.2008.00602.x

Khalili, 2013, Iran. J. Reprod. Med., 11, 1

Kikuchi, 2000, Biol. Reprod., 63, 715, 10.1095/biolreprod63.3.715

Koyama, 2014, J. Reprod. Dev., 60, 136, 10.1262/jrd.2013-115

Koyama, 2014, J. Reprod. Dev., 60, 136, 10.1262/jrd.2013-115

Kujjo, 2012, Reproduction, 143, 1, 10.1530/REP-11-0350

Lacham-Kaplan, 2008, Reprod. Biol. Endocrinol., 6, 58, 10.1186/1477-7827-6-58

Lee, 2014, Asian-australas. J. Anim. Sci., 27, 635, 10.5713/ajas.2013.13816

Liang, 2016, Theriogenology, 85, 754, 10.1016/j.theriogenology.2015.10.021

Liu, 2011, Reprod. Biol. Endocrinol., 9, 149, 10.1186/1477-7827-9-149

Livak, 2001, methods, 25, 402, 10.1006/meth.2001.1262

Lord, 2013, Biol. Reprod., 88, 67, 10.1095/biolreprod.112.106450

Lu, 2011, Theriogenology, 76, 967, 10.1016/j.theriogenology.2011.04.026

Luo, 2009, Mol. Reprod. Dev., 76, 819, 10.1002/mrd.21034

McGinnis, 2014, Mol. Reprod. Dev., 81, 928, 10.1002/mrd.22413

Miao, 2009, Hum. Reprod. Update, 15, 573, 10.1093/humupd/dmp014

Nasr-Esfahani, 1990, Development, 109, 501, 10.1242/dev.109.2.501

Niwa, 1993, J. Reprod. Fertil. Suppl., 48, 49

Papis, 2007, J. Pineal Res., 43, 321, 10.1111/j.1600-079X.2007.00479.x

Park, 2005, Theriogenology, 64, 123, 10.1016/j.theriogenology.2004.11.012

Picton, 2002, Curr. Opin. Obstet. Gynecol., 14, 295, 10.1097/00001703-200206000-00009

Pieri, 1995, Arch. Gerontol. Geriatr., 20, 159, 10.1016/0167-4943(94)00593-V

Ptak, 2006, Theriogenology, 65, 1891, 10.1016/j.theriogenology.2005.10.018

Reiter, 2010, Prog. Brain Res., 181, 127, 10.1016/S0079-6123(08)81008-4

Rivara, 2005, J. Med. Chem., 48, 4049, 10.1021/jm048956y

Romero, 2014, J. Pineal Res., 56, 343, 10.1111/jpi.12132

Roth, 2005, Reproduction, 129, 235, 10.1530/rep.1.00394

Ruder, 2008, Hum. Reprod. Update, 14, 345, 10.1093/humupd/dmn011

Shojo, 2000, J. Mamm. Ova Res., 17, 35, 10.1274/jmor.17.35

Slotte, 1990, Hum. Reprod., 5, 319, 10.1093/oxfordjournals.humrep.a137097

Somfai, 2011, Anat. Histol. Embryol., 40, 335, 10.1111/j.1439-0264.2011.01079.x

Stojkovic, 2001, Biol. Reprod., 64, 904, 10.1095/biolreprod64.3.904

Sun, 2012, Fertil. Steril., 97, 984, 10.1016/j.fertnstert.2012.01.101

Takahashi, 2009, Biol. Reprod., 80, 493, 10.1095/biolreprod.108.072017

Tamura, 2008, J. Pineal Res., 44, 280, 10.1111/j.1600-079X.2007.00524.x

Tamura, 2012, J. Ovarian Res., 5, 5, 10.1186/1757-2215-5-5

Tang, 2013, Biol. Reprod., 89, 124, 10.1095/biolreprod.113.111302

Tar�n, 2000, Hum. Reprod. Update, 6, 532, 10.1093/humupd/6.6.532

Tian, 2014, J. Pineal Res., 57, 239, 10.1111/jpi.12163

Tiwari, 2016, Reactive Oxygen Species, 1, 110, 10.20455/ros.2016.817

Tripathi, 2010, J. Cell. Physiol., 223, 592, 10.1002/jcp.22108

Wang, 2014, J. Pineal Res., 56, 333, 10.1111/jpi.12126

Ward, 2002, Theriogenology, 57, 2105, 10.1016/S0093-691X(02)00696-9

Wignall, 2009, Nat. Cell Biol., 11, 839, 10.1038/ncb1891

Wilding, 2001, Hum. Reprod., 16, 909, 10.1093/humrep/16.5.909

Y?ce, 2001, Dev. Biol., 237, 29, 10.1006/dbio.2001.0361

Zhao, 2011, Fertil. Steril., 95, 2786, 10.1016/j.fertnstert.2011.04.089

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Reproduction, Fertility and Development

Tập 29 Số 9

1821

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