Evaluation of antimotility effect of Lantana camara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice
Tóm tắt
Lantana camara L. (Verbenaceae), a widely growing shrub which is toxic to some animal species, has been used in the traditional medicine for treating many ailments. The purpose of the present study was to evaluate the antimotility effects of Lantana camara leaf constituents in mice intestine. Evaluation of antimotility activity was done in intestine of mice treated with Lantana camara leaf powder, Lantana camara methanolic extract (LCME), lantadene A, neostigmine and neostigmine + LCME. Neostigmine was used as a promotility agent. Intestinal motility was assessed by charcoal meal test and gastrointestinal transit rate was expressed as the percentage of the distance traversed by the charcoal divided by the total length of the small intestine. The antidiarrheal effect of LCME was studied against castor oil induced diarrhea model in mice. The intestinal transit with LCME at a dose of 500 mg/kg was 26.46% whereas the higher dose (1 g/kg) completely inhibited the transit of charcoal in normal mice. The % intestinal transit in the neostigmine pretreated groups was 24 and 11 at the same doses respectively. When the plant extracts at 125 and 250 mg/kg doses were administered intraperitonealy, there was significant reduction in fecal output compared with castor oil treated mice. At higher doses (500 and 1000 mg/kg), the fecal output was almost completely stopped. The remarkable antimotility effect of Lantana camara methanolic extract against neostigmine as promotility agent points towards an anticholinergic effect due to Lantana camara constituents and attests to its possible utility in secretory and functional diarrheas and other gastrointestinal disorders. This effect was further confirmed by significant inhibition of castor oil induced diarrhea in mice by various doses of LCME.
Tài liệu tham khảo
Massee B, McGahey C: A Framework for Action; Child Diarrhea Prevention. Global healthlink, a publication of the Global Health Council, www.globalhealth.org. 2001, 111
Harrison TR: Diarrhea and Constipation. Principles of internal medicine. 2005, New York: Macgraw Hill, 224-232. 16
Hardman JG, Limbird LE: Goodman's and Gilman's: Drugs affecting gastrointestinal function. The pharmacological basis of therapeutics. 2001, New York: Macgraw Hill, 1023-1024. 10
Chitme HR, Chandra R, Kaushik S: Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br. in experimental animals. J Pharm Pharmaceut Sci. 2004, 7: 70-75.
Sharma OP, Makkar HPS, Dawra RK: A review of the noxious plant Lantana camara. Toxicon. 1988, 26: 975-987. 10.1016/0041-0101(88)90196-1.
Sharma OP, Makkar HPS: Lantana – the foremost livestock killer in Kangra district of Himachal Pradesh. Livestock Adv. 1981, 6: 29-31.
Sharma OP, Dawra RK, Makkar HPS: Toxicity of isolated lantana (Lantana camara L.) constituents to male and female guinea pigs. Vet Hum Toxicol. 1989, 31: 10-13.
Ghisalberti EL: Lantana camara Linn. (Review). Fitoterapia. 2000, 71: 467-485. 10.1016/S0367-326X(00)00202-1.
Duke , James A: Handbook of phytochemical constituents of GRAS herbs and other economic plants. 1992, Boca Raton, FL. CRC Press
Shashi BM, Niranjan PS, Subodh KR, Sharma OP: Potential Antitumor Agents fromf Lantana camara: Structures of Flavonoid and Phenylpropanoid Glycosides. Tetrahedron. 1994, 50: 9439-9446. 10.1016/S0040-4020(01)85518-6.
O' Neill MJ, Lewis JA, Noble HM, Holland S, Mansat C, Farthing JE, Foster G, Noble D, Lane SJ, Sidebottom PJ, Lynn SM, Hayes MV, Dix CJ: Isolation of translactone-containing triterpenes with thrombin inhibitory activities from the leaves of lantana camara . J Nat Prod. 1998, 61: 1328-1331. 10.1021/np970464j.
Uzcategui B, Avila D, Heberto SR, Quintero L, Ortega J, Gonzalez YB: Anti-inflammatory, antinociceptive and antipyretic effects of Lantana trifolia Linnaeus in experimental animals. Investigacion Clinica. 2004, 45: 4-
Barton DHR, De Mayo P, Orr JC: Triterpenoids Part XXIII: The nature of lantadene A. J Chem Soc. 1956, 4160-4162. 10.1039/jr9560004160.
Sharma S, Singh A, Sharma OP: An improved procedure for isolation and purification of lantadene A, the bioactive pentacyclic triterpenoid from Lantana camara leaves. Journal of Medicinal and Aromatic Plant Sciences. 1999, 21: 686-688.
Jabbar S, Khan MTH, Choudhuri MSK, Gafur MA, Ahmad K: Effect of Semicarpus anacardium Linn. on acute experimental diarrhea. Ham Med. 1999, XLII: 48-53.
Awouters F, Niemegeers CJE, Lenaerts FM, Janseen PAJ: Delay of castor oil diarrhea in rats; a new way to evaluate inhibitors of prostaglandin biosynthesis. Journal of Pharmacy Pharmacology. 1978, 30: 41-45.
Lundregen O: Enteric nerves and diarrhea. Pharmacology and Toxicology. 2002, 90: 109-120. 10.1034/j.1600-0773.2002.900301.x.
Korman LY: Secretory and miscellaneous noninfectious diarrhea. A Pharmacologic Approach to gastrointestinal disorders. Edited by: Lewis JH. 1994, Williams and Wilkins, Baltimore, 281-291.
Furness JB, Costa M: The enteric nervous system. 1987, Churchill Livingstone, New York
Iwao I, Terada Y: On the mechanism of diarrhea due to castor oil. Jpn J Pharmacol. 1962, 12: 137-145.
Watson WC, Gordan RS: Studies on the digestion absorption and metabolism of castor oil. Biochem pharmacol. 1962, 11: 229-236. 10.1016/0006-2952(62)90078-3.
Uchida M, Kato Y, Matsuede K, Shode R, Muraoka A, Yemato S: Involvement of NO from nerves in diarrhea induced by castor oil in rats. Jpn J Pharmacol. 2000, 82: 168-170. 10.1254/jjp.82.168.
Saito T, Mizutani F, Iwanaga Y, Morikawa K, Kato H: Laxative and anti-diarrheal activity of polycarbophil in mice and rats. Jpn J Pharmacol. 2002, 89: 133-141. 10.1254/jjp.89.133.
Izzo AA, Gaginella TS, Mascolo N, Capasso S: Recent findings on the mode of action of laxatives: the role of platelet activating factor and nitric oxide. Trends Pharmacol Sci. 1998, 19: 403-405. 10.1016/S0165-6147(98)01249-8.
Kellerman TS, Coetzer JAW, Naude TW: Plant poisoning and mycotoxicosis of livestock in Southern Africa. 1988, Oxford University Press, Cape Town
Akhter MH, Mathur M, Bhide NK: Skin and liver toxicity in experimental Lantana camara poisoning in albino rats. Indian J Physiol Pharmacol. 1990, 34: 13-16.
Pass MA, Seawright AA, Heath TJ: Lantadene toxicity in sheep. A model for cholestasis. Pathology. 1979, 11: 89-94.
Dhillon KS, Paul BS: Clinical studies of Lantana camara L. poisoning in buffalo calves with special reference to its effect on rumen motility. Indian J Animal Sci. 1971, 41: 945-948.
Sweeney MC, Pass MA: The mechanism of ruminal stasis in lantana poisoned sheep. Q J Exp Physiol Cogn Med Sci. 1983, 301-314.
Holson D, Oster N: Constipation. eMedicine. 2001, 2: S1-9.
Bruns John: Toxicity, Anticholinergic. eMedicine. 2001, 2: S1-10.
Longanga_Otshudi A, Vercruysse A, Foriers A: Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhea in Lomela area, Democratic Republic of Congo (DRC). J Ethnopharmacol. 2000, 71: 411-423. 10.1016/S0378-8741(00)00167-7.
Heinrich M, Robles M, West JE, Ortiz de Montellano BR, Rodriguez E: Ethnopharmacology of Mexican asteraceae (Compositae). Annu Rev Pharmacol Toxicol. 1998, 38: 539-565. 10.1146/annurev.pharmtox.38.1.539.
Pan WD, Mai LT, Li YJ, Xu XL, Yu DQ: Studies on the chemical constituents of the leaves of Lantana camara. Yao Xue Xue Bao. 1993, 28: 35-39.
Begum S, Wahab A, Siddiqui BS: Pentacyclic triterpenoids from the aerial parts of Lantana camara. Chem Pharm Bull. 2003, 51: 134-137. 10.1248/cpb.51.134.
Ahmed ZF, Shoaib AM, Wassel GM, Sayyad SM: Phytochemical study of Lantana camara L . Planta Med. 1972, 21: 282-288.
Herbert JM, Maffrand JP, Taoubi K, Augereau JM, Fouraste I, Gleye J: Verbascoside isolated from Lantana camara, an inhibitor of protein kinase C. J Nat Prod . 1991, 54: 1595-600. 10.1021/np50078a016.
Sales ME, Sterin-Borda L, de Bracco MM, Borda ES: Tyrosine kinase regulatory action on ileal muscarinic effects of IFN-gamma. J Interferon Cytokine Res. 1999, 19: 375-382. 10.1089/107999099314081.
Nikiema JB, Vanhaelen Fastre R, Vanhaelen M, Fontaine J, De Graef C, Heenen M: Effects of anti-inflammatory triterpenes isolated from Leptadenia hastate latex on keratinocyte proliferation. Phytother Res. 2001, 15: 131-134. 10.1002/ptr.700.
Pass MA, Heath TJ: The effect of Lantana camara on intestinal motility in sheep. J Comp Path. 1978, 88: 149-156. 10.1016/0021-9975(78)90071-3.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/5/18/prepub