A Novel Gene of HIV-1, vpu , and Its 16-Kilodalton Product

American Association for the Advancement of Science (AAAS) - Tập 241 Số 4870 - Trang 1221-1223 - 1988
Klaus Strebel1, Thomas Klimkait1, María Ángeles Martín1
1Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

Tóm tắt

A 16-kilodalton protein expressed in cells producing the human immunodeficiency virus (HIV-1) was identified as the gene product of the vpu open reading frame. When expressed in vitro, the 81-amino acid vpu protein reacted with about one-third of the serum samples from AIDS patients that were tested, indicating that the vpu open reading frame is expressed in vivo as well. Introduction of a frame-shift mutation into the vpu open reading frame did not significantly interfere with expression of the major viral proteins in a transient expression system. However, a five- to tenfold reduction in progeny virions was observed after the infection of T lymphocytes with the mutant virus. These data suggest that the vpu gene product is required for efficient virus replication and may have a role in assembly or maturation of progeny virions.

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American Association for the Advancement of Science (AAAS)

Tập 241 Số 4870

1221-1223

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