Frequent Detection and Isolation of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and at Risk for AIDS

American Association for the Advancement of Science (AAAS) - Tập 224 Số 4648 - Trang 500-503 - 1984
Robert C. Gallo1, S. Zaki Salahuddin1, Mikuláš Popovič1, Gene M. Shearer2, Mark H. Kaplan3, Barton F. Haynes4, T J Palker4, Robert R. Redfield5, James M. Oleske6, Bijan Safai7, Gilbert White8, Paul S. Foster8, Phillip D. Markham9
1Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20205
2Immunology Branch, National Cancer Institute
3Division of Infectious Diseases, North Shore University Hospital, Manhusset, New York 11030
4Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710
5Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, D.C. 20012
6Division of Allergy, Immunology, and Infectious Disease, University of Medicine and Dentistry of New Jersey, Newark 07103
7Dermatology Service, Memorial Sloan Kettering Cancer Center, New York 10021
8Department of Medicine, University of North Carolina, Chapel Hill 27514.
9Department of Cell Biology, Litton Bionetics, Inc., Kensington, Maryland 20895

Tóm tắt

Peripheral blood lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS) were grown in vitro with added T-cell growth factor and assayed for the expression and release of human T-lymphotropic retroviruses (HTLV). Retroviruses belonging to the HTLV family and collectively designated HTLV-III were isolated from a total of 48 subjects including 18 of 21 patients with pre-AIDS, three of four clinically normal mothers of juveniles with AIDS, 26 of 72 adult and juvenile patients with AIDS, and from one of 22 normal male homosexual subjects. No HTLV-III was detected in or isolated from 115 normal heterosexual subjects. The number of HTLV-III isolates reported here underestimates the true prevalence of the virus since many specimens were received in unsatisfactory condition. Other data show that serum samples from a high proportion of AIDS patients contain antibodies to HTLV-III. That these new isolates are members of the HTLV family but differ from the previous isolates known as HTLV-I and HTLV-II is indicated by their morphological, biological, and immunological characteristics. These results and those reported elsewhere in this issue suggest that HTLV-III may be the primary cause of AIDS.

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American Association for the Advancement of Science (AAAS)

Tập 224 Số 4648

500-503

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