Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

American Association for the Advancement of Science (AAAS) - Tập 333 Số 6040 - Trang 348-353 - 2011
Farren J. Isaacs1, Peter A. Carr2,3, Harris H. Wang1,4,5,6, Marc J. Lajoie1,7, Bram Sterling2,3, Laurens Kraal1, Andrew C. Tolonen1, Tara A. Gianoulis1,6, Daniel B. Goodman1,5, Nikos B. Reppas8, Christopher J. Emig9, Duhee Bang10, Samuel James Hwang11, Michael C. Jewett12,1, Joseph M. Jacobson2,3, George M. Church1,6
1Department of Genetics, Harvard Medical School, Boston, MA 02115 USA
2Center for Bits and Atoms, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
3MIT Media Lab, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
4Program in Biophysics, Harvard University, Cambridge, MA 02138, USA
5Program in Medical Engineering and Medical Physics, Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
6Wyss Institute, Harvard University, Cambridge, MA 02115, USA.
7Program in Chemical Biology, Harvard University, Cambridge, MA 02138, USA
8Joule Unlimited, Cambridge, MA 02139, USA.
9Department of Bioengineering, Stanford University, Palo Alto, CA 94305, USA.
10Department of Chemistry, Yonsei University, Shinchon 134, Seoul 120-749, Korea
11Department of Materials Science And Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
12Department of Chemical and Biological Engineering and Chemistry of Life Processes Institute, Northwestern University, Evanston, IL 60208, USA.

Tóm tắt

Template-mediated, genome construction and assembly created a strain with 80 precise codon changes.

Từ khóa


Tài liệu tham khảo

10.1038/nchembio847

10.1038/35047500

10.1074/jbc.R109.091306

10.1038/nature08817

10.1126/science.1144622

10.1038/nbt.1590

10.1126/science.1126439

Chan L. Y. Kosuri S. Endy D. Mol. Syst. Biol. 1 0018 (2005).

10.1073/pnas.0503868102

10.1126/science.1151721

10.1126/science.1144622

10.1126/science.1190719

10.1126/science.1155761

10.1038/nature08187

10.1074/jbc.270.39.22801

10.1073/pnas.86.10.3699

10.1073/pnas.121164898

10.1073/pnas.2434959100

10.1128/JB.185.19.5673-5684.2003

10.1101/gr.2.1.14

10.1093/nar/gnh160

10.1016/j.virol.2003.11.007

10.1093/genetics/154.3.959

10.1038/35012500

10.1016/0092-8674(91)90205-D

10.1073/pnas.100127597

10.1093/nar/gkm1084

10.1093/nar/gni035

10.1128/JB.01448-06

10.1073/pnas.84.17.6220

10.1093/bioinformatics/btp324

10.1093/bioinformatics/btp352

10.1093/bioinformatics/btq330

10.1128/JB.00872-09

10.1093/nar/gkn395

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 333 Số 6040

348-353

Thông tin tác giả