Damage to microsomal membrane by lipid peroxidation

Lipids - Tập 8 Số 4 - Trang 177-182 - 1973
Wayne R. Bidlack1, Al L. Tappel1
1Department of Food Science and Technology, University of California, Davis

Tóm tắt

AbstractAlterations of microsomal membrane integrity were examined during lipid peroxidation. Loss of membrane‐bound NADPH cytochromec reductase activity and protein release into the aqueous phase were related to disruption of the lipophilic region of the membrane. Formation of fluorescent products in the lipid phase of the membrane occurred only in the presence of peroxidation products. Changes in the membrane lipid phase during peroxidation included a decrease in the reactive amino groups of the phospholipids and a decrease in detectable phosphatidyl ethanolamine. In an ultrafiltration continuous flow chamber, peroxidized microsomal membranes were hydrolyzed to a lesser degree by solubilized lysosomal cathepsins and to a greater degree by lysosomal nucleases than were nonperoxidized membranes.

Từ khóa


Tài liệu tham khảo

10.1007/BF02541295

Tappel A.L., Pathological Aspects of Cell Membranes

10.1016/0003-9861(66)90168-8

10.1007/BF02536377

Menzel D.B., 1967, Ibid., 2, 83

10.1021/bi00835a020

10.1042/bj1230983

10.1007/BF02531978

10.1007/BF02609496

10.1016/S0021-9258(18)93474-5

10.1007/BF02531045

Branton D. andD.W.Deamer Protoplasmatologiall/E/l:1(1972).

10.1248/cpb.19.385

Perkins E.G., 1965, Metabolism of Lipids as Related to Atherosclerosis, 48

10.1111/j.1532-5415.1969.tb02286.x

Tappel A.L., 1968, Geriatrics, 23, 97

Zeman W., 1971, Advan. Gerontol. Res., 3, 147

10.1016/0014-4800(69)90069-0

Recknagel R.O., 1967, Pharm. Rev., 19, 145

10.1042/bj0600604

10.1111/j.1365-2621.1963.tb01666.x

10.1007/BF02531316

10.1021/ac60149a611

10.1016/S0076-6879(55)02287-8

Dallner G., 1963, Acta Pathol. Microbiol. Scand. Suppl., 166, 1

Fiske C.H., 1957, Methods in Enzymology, 843

10.1021/bi00869a021

Ansorge S., 1971, Acta Biol. Med. Ger., 26, 953

10.1016/S0021-9258(18)94295-X

Litwack G., 1966, Experimental Biochemistry, 21

Hochstein P., 1964, CIBA Symposium on Cellular Injury, 123, 10.1002/9780470719336.ch7

10.1016/0003-9861(65)90025-1

10.1007/BF02531296

Bidlack W.R., 1972, Ph.D. thesis

10.1111/j.1749-6632.1972.tb27851.x

10.1007/BF02531333

10.1093/jn/100.4.413

Porta E.A., 1969, Pigments in Pathology, 191

10.1021/bi00835a019

10.1126/science.175.4021.530

Thông tin
Thông tin xuất bản

Lipids

Tập 8 Số 4

177-182

Thông tin tác giả