Lymphocyte Adhesion to Human Endothelial Cells Induces Tissue Factor Expression via a Juxtacrine Pathway

Thrombosis and Haemostasis - Tập 73 Số 03 - Trang 421-428 - 1995
E. D. Schmid1, Thomas Müller1, Ralph Budzinski2, Klaus Pfizenmaier3, Klaus Binder1
1The Department of Pharmacological Research, Dr. Karl Thomae GmbH, Biberach/Riß, Germany
2Department of Biochemical Research, Dr. Karl Thomae GmbH, Biberach/Riß, Germany
3Institute for Cell Biology and Immunology, University of Stuttgart, Stuttgart, Germany

Tóm tắt

SummaryTo study the effect of lymphocyte adhesion on the procoagulant activity of endothelial cells, we have stimulated HUVECs with interferon-γ to upregulate adhesion molecules. Subsequent addition of lymphocytes induced the expression of tissue factor (TF) by HUVECs. Both CD4+ and CD8+ T-cells promoted this TF synthesis via distinct adhesion molecules (CD4+ T-cells: E-selectin and ICAM-1; CD8+ T-cells: MHC-I molecules). In addition, tumor necrosis factor-α and -β (TNFα, TNFβ) and platelet-activating factor (PAF) were involved in lymphocyte-mediated TF expression on HUVECs. We demonstrate that PAF plays a pivotal role in this process. Adhesion of lymphocytes to endothelial cell surface molecules induced the release of PAF. PAF, in turn, caused the production of T.NFα and TNFβ, both of which are potent stimulators of TF expression.

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Thrombosis and Haemostasis

Tập 73 Số 03

421-428

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