Human NK cells display major phenotypic and functional changes over the life span

Aging Cell - Tập 9 Số 4 - Trang 527-535 - 2010
Magali Le Garff‐Tavernier1,2,3, Vivien Béziat2,3, Julie Decocq2,4,3, Virginie Siguret5,6, Frédérique Gandjbakhch7,3, Stéphane Lehéricy8, Patrice Debré2,3, Hélène Merle‐Béral1,9,3, Vincent Vieillard2,3
1AP-HP, Hôpital Pitié-Salpêtrière, Service d’Hématologie Biologique, F-75013, Paris, France
2INSERM, UMR-S 945, F-75013, Paris, France
3UPMC Université Paris 06, F-75005 Paris, France
4Laboratoire français de fractionnement et des biotechnologies (LFB), F-91940, Les Ulis, France
5AP-HP, Hôpital Charles-Foix, Laboratoire d’Hématologie, F-94205, Ivry sur Seine, France
6Université Paris Descartes, F-75006 Paris, France
7AP-HP, Hôpital Pitié-Salpêtrière, Service de Rhumatologie, F-75013, Paris, France
8Hôpital Charles-Foix, AP-HP, Service de Gériatrie, F-94205, Ivry sur Seine, France
9Centre des Cordeliers, INSERM UMR-S 872, F-75006, Paris, France

Tóm tắt

SummaryAging is generally associated with an increased predisposition to infectious diseases and cancers, related in part to the development of immune senescence, a process that affects all cell compartments of the immune system. Although many studies have investigated the effects of age on natural killer (NK) cells, their conclusions remain controversial because the diverse health status of study subjects resulted in discordant findings. To clarify this situation, we conducted the first extensive phenotypic and functional analysis of NK cells from healthy subjects, comparing NK cells derived from newborn (cord blood), middle‐aged (18–60 years), old (60–80 years), and very old (80–100 years) subjects. We found that NK cells in cord blood displayed specific features associated with immaturity, including poor expression of KIR and LIR‐1/ILT‐2 and high expression of both NKG2A and IFN‐γ. NK cells from older subjects, on the other hand, preserved their major phenotypic and functional characteristics, but with their mature features accentuated. These include a profound decline of the CD56bright subset, a specific increase in LIR‐1/ILT‐2, and a perfect recovering of NK‐cell function following IL2‐activation in very old subjects. We conclude that the preservation of NK cell features until very advanced age may contribute to longevity and successful aging.

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