Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients

Springer Science and Business Media LLC - Tập 11 - Trang 1-14 - 2014
Lavanya Balakrishnan1,2, Mitali Bhattacharjee1,3, Sartaj Ahmad1,4, Raja Sekhar Nirujogi1,5, Santosh Renuse1,3, Yashwanth Subbannayya1,6, Arivusudar Marimuthu1, Srinivas M Srikanth1,5, Rajesh Raju1, Mukesh Dhillon7, Navjyot Kaur7, Ramesh Jois8, Vivek Vasudev9, YL Ramachandra2, Nandini A Sahasrabuddhe1, TS Keshava Prasad1,3,4, Sujatha Mohan10, Harsha Gowda1, Subramanian Shankar7, Akhilesh Pandey11,12,13,14
1Institute of Bioinformatics, International Technology Park, Bangalore, India
2Department of Biotechnology, Kuvempu University, Shankaraghatta, India
3Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Kollam, India
4Manipal University, Manipal, India
5Centre for Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, India
6Rajiv Gandhi University of Health Sciences, Bangalore, India
7Department of Internal Medicine, Armed Forces Medical College, Pune, India
8Department of Rheumatology, Fortis Hospitals, Bangalore, India
9Department of Rheumatology, Command Airforce Hospital, Bangalore, India
10Laboratory for Integrated Bioinformatics, RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Yokohama Institute, Kanagawa, Japan
11McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, USA
12Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, USA
13Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, USA
14Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, USA

Tóm tắt

Rheumatoid arthritis and osteoarthritis are two common musculoskeletal disorders that affect the joints. Despite high prevalence rates, etiological factors involved in these disorders remain largely unknown. Dissecting the molecular aspects of these disorders will significantly contribute to improving their diagnosis and clinical management. In order to identify proteins that are differentially expressed between these two conditions, a quantitative proteomic profiling of synovial fluid obtained from rheumatoid arthritis and osteoarthritis patients was carried out by using iTRAQ labeling followed by high resolution mass spectrometry analysis. We have identified 575 proteins out of which 135 proteins were found to be differentially expressed by ≥3-fold in the synovial fluid of rheumatoid arthritis and osteoarthritis patients. Proteins not previously reported to be associated with rheumatoid arthritis including, coronin-1A (CORO1A), fibrinogen like-2 (FGL2), and macrophage capping protein (CAPG) were found to be upregulated in rheumatoid arthritis. Proteins such as CD5 molecule-like protein (CD5L), soluble scavenger receptor cysteine-rich domain-containing protein (SSC5D), and TTK protein kinase (TTK) were found to be upregulated in the synovial fluid of osteoarthritis patients. We confirmed the upregulation of CAPG in rheumatoid arthritis synovial fluid by multiple reaction monitoring assay as well as by Western blot. Pathway analysis of differentially expressed proteins revealed a significant enrichment of genes involved in glycolytic pathway in rheumatoid arthritis. We report here the largest identification of proteins from the synovial fluid of rheumatoid arthritis and osteoarthritis patients using a quantitative proteomics approach. The novel proteins identified from our study needs to be explored further for their role in the disease pathogenesis of rheumatoid arthritis and osteoarthritis. Sartaj Ahmad and Raja Sekhar Nirujogi contributed equally to this article.

Tài liệu tham khảo

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