A blue-light photoreceptor mediates the fluence-rate-dependent expression of genes encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase in light-grown Phaseolus vulgaris primary leaves
Tóm tắt
The level of transcripts of genes encoding the small subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcS genes) in the primary leaves of Phaseolus vulgaris L. increases substantially when plants grown in a low fluence rate of white light (15 μmol m−2 s−1; 400–700 nm) are transferred to a tenfold higher fluence rate of identical spectral quality. To investigate which photoreceptor acts as the fluence-rate detector, plants grown for 16 d in low white light were transferred to blue-enriched or red light environments of various fluence rates. The results indicate that the fluence-rate-dependent increase in rbcS expression is mediated specifically by blue light. Red light of the same fluence rate, which was found to be equally effective in driving photosynthesis, had much less effect on expression, indicating that light absorbed by the photosynthetic pigments does not mediate the response. Moreover, there is no correlation of the transcript levels with either the cycling rate or photoequilibrium of the phytochrome system. Run-on assays with isolated nuclei indicate that blue light substantially increases the rate of rbcS transcription. Experiments with gene-specific probes show that individual members of the P. vulgaris rbcS gene family exhibit the fluence-rate-dependent, blue-light-mediated increase in expression.
Tài liệu tham khảo
Anderson, J.M. (1986) Photoregulation of the composition, function structure of thylakoid membranes. Annu. Rev. Plant Physol. 37, 93–136
Anderson, J.M., Chow, W.S., Goodchild, D.J. (1988) Thylakoid membrane organisation in sun/shade acclimation. Austr. J. Plant Physiol. 15, 11–26
Barnett, L.K., Clugston, C.K., Jenkins, G.I. (1987) Two phyto-chrome-mediated effects of light on transcription of genes encoding the small subunit of ribulose 1,5 bisphosphate carboxylase-oxygenase in dark-grown pea (Pisum sativum) plants. FEBS Letts. 224, 287–290
Beggs, C.J., Holmes, M.G., Jabben, M., Schäfer, E. (1980) Action spectra for the inhibition of hypocotyl growth by continuous irradiation in light and dark-grown Sinapis alba L. seedlings. Plant Physiol. 66, 615–618
Berry-Lowe, S.L., Meagher, R.B. (1985) Transcriptional regulation of a gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean tissue is linked to the phytochrome response. Mol. Cell. Biol. 5, 1910–1917
Björkman, O. (1981) Responses to different quantum flux densities. In: Encyclopedia of plant physiology. N.S., vol. 12A, pp. 57–107, Lange, O.L., Nobel, P.S., Osmond, C.B., Zeigler, H., eds. Springer, Berlin
Boardman, N.K. (1977) Comparative photosynthesis of sun and shade plants. Annu. Rev. Plant Physiol. 28, 355–377
Chory, J. (1991) Light signals in leaf and chloroplast development: photoreceptors and downstream responses in search of a transduction pathway. New Biol. 3, 538–548
Chory, J., Peto, C.A., Ashbaugh, M., Saganich, R., Pratt, L., Ausubel, F. (1989) Different roles for phytochrome in etiolated and green plants deduced from characterisation of Arabidopsis thaliana mutants. Plant Cell 1, 867–880
Chow, W.S., Goodchild, D.J., Miller, C., Anderson, J.M. (1990) The influence of high levels of brief or prolonged supplementary far-red illumination during growth on the photosynthetic characteristics, composition and morphology of Pisum sativum chloroplasts. Plant Cell Environ. 13, 135–145
Clugston, C.K., Barnett, L.K., Urwin, N.A.R., Jenkins, G.I. (1990) Photoreceptors controlling transcription of rbcS genes in green leaf tissue of Pisum sativum. Photochem. Photobiol. 52, 23–28
Crookston, R.K., Treharne, K.J., Ludford, P., Ozbun, J.L. (1975) Response of beans to shading. Crop Sci. 15, 412–416
Delieu, T., Walker, D.A. (1981) Polarographic measurement of photosynthetic oxygen evolution by leaf discs. New Phytol. 89, 165–178
Drumm-Herrel, H., Mohr, H. (1988) Mode of coaction between UV-A and light absorbed by phytochrome in control of appearance of ribulose-1,5-bisphosphate carboxylase in the shoot of milo (Sorghum vulgare Pers.). Photochem. Photobiol. 47, 599–604
Eskins, K., Beremand, P.D. (1990) Light-quality and irradiance-level control of light-harvesting complex of photosystem 2 in maize mesophyll cells. Evidence for a low fluence-rate threshold in blue-light reduction of mRNA and protein. Physiol. Plant. 78, 435–440
Fluhr, R., Chua, N-H. (1986) Developmental regulation of two genes encoding ribulose-bisphosphate carboxylase small subunit in pea and transgenic petunia plants: phytochrome response and blue light induction. Proc. Natl. Acad. Sci. USA 83, 2358–2362
Frosch, S., Bergfeld, R., Mehnert, C., Wagner, E., Greppin, H. (1985) Ribulose bisphosphate carboxylase capacity and Chlorophyll content in developing seedlings of Chenopodium rubrum L. growing under light of different qualities and fluence rates. Photosynth. Res. 7, 41–67
Gaba, V., Black, M., Attridge, T.H. (1984) Photocontrol of hypocotyl elongation in de-etiolated Cucumis sativus L. Plant Physiol. 74, 897–900
Gallagher, T.F., Ellis, R.J. (1982) Light-stimulated transcription of genes for two chloroplast polypeptides in isolated pea leaf nuclei. EMBO J. 1, 1493–1498
Gamble, P.E., Mullet, J.E. (1989) Blue light regulates the accumulation of two psbD-psbC transcripts in barley chloroplasts. EMBO J. 8, 2785–2794
Gardner, G., Graceffo, M.A. (1982) The use of a computerized spectroradiometer to predict phytochrome photoequilibria under polychromatic irradiation. Photochem. Photobiol. 36, 349–354
Gilmartin, P.M., Sarokin, L., Memelink, J., Chua, N-H. (1990) Molecular light switches for plant genes. Plant Cell 2, 369–378
Glazer, A.N., Melis, A. (1987) Photochemical reaction centers: structure, organization and function. Annu. Rev. Plant Physiol. 38, 11–45
Hartmann, K.M. (1966) A general hypothesis to interpret “high energy phenomena” of photomorphogenesis on the basis of phytochrome. Photochem. Photobiol. 5, 349–366
Holmes, M.G., Schäfer, E. (1981) Action spectra for changes in the “high irradiance reaction” in hypocotyls of Sinapis alba L. Planta 153, 267–272
Holmes, M.G., Wagner, E. (1981) Phytochrome control of hypocotyl extension in light-grown Chenopodium rubrum. Physiol. Plant. 53, 233–238
Jenkins, G.I. (1986) Photoregulation of expression of genes encoding ribulose-1,5-bisphosphatecarboxylase/oxygenase. Biochem. Soc. Trans. 14, 22–25
Jenkins, G.I. (1991) Photoregulation of plant gene expression. In: Developmental regulation of plant gene expression, pp. 1–41, Grierson, D., ed. Blackie, Glasgow
Jenkins, G.I., Smith, H. (1985) Red: far ratio does not modulate the abundance of transcripts for two major chloroplast polypeptides in light-grown Pisum sativum terminal shoots. Photochem. Photobiol. 42, 679–684
Jordan, B.R., Hopley, J.G., Thompson, W.F. (1989) Chloroplast gene expression in lettuce grown under different irradiances. Planta 178, 69–75
Karlson, P.E. (1986) Blue light regulation of stomata in wheat seedlings. II. Action spectrum and search for action dichroism. Physiol. Plant. 66, 207–210
Knight, M.R., Jenkins, G.I. (1992) Genes encoding the small sub-unit of ribulose 1,5-bisphosphate carboxylase/oxygenase in Phaseolus vulgaris L.: nucleotide sequence of cDNA clones and initial studies of expression. Plant Mol. Biol. 18, 567–579
Leong, T.Y., Anderson, J.M. (1986) Light-quality and irradiance adaptation of the composition and function of pea-thylakoid membranes. Biochim. Biophys. Acta 850, 57–63
López-Juez, E., Buurmeijer, W.F., Heeringa, G.H., Kendrick, R.E., Wesselius, J.C. (1990a) Response of light-grown wild-type and long hypocotyl mutant cucumber plants to end-of-day far-red light. Photochem. Photobiol. 52, 143–149
López-Juez, E., Nagatani, A., Buurmeijer, W.F., Peters, J., Furuya, M., Kendrick, R.E., Wesselius, J.C. (1990b) Response of lightgrown wild-type and aurea-mutant tomato plants to end-of-day far-red light. J Photochem. Photobiol. B: Biol 4, 391–405
López-Juez, E., Nagatani, A., Tomizawa, K-I, Deak, M., Kern, M., Kendrick, R.E., Furuya, M. (1992) The cucumber long hypocotyl mutant lacks a light-stable PHYB-like phytochrome. Plant Cell 4, 241–251
Marrs, K.A., Kaufman, L.S. (1989) Blue-light regulation of transcription for nuclear genes in pea. Proc. Natl. Acad. Sci. USA 86, 492–495
Maxam, A.M., Gilbert, W. (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. Methods Enzymol. 65, 499–560
Oelmüller, R., Kendrick, R.E., Briggs, W.R. (1989) Blue-light mediated accumulation of nuclear-encoded transcripts coding for proteins of the thylakoid membrane is absent in the phyto-chrome-deficient aurea mutant of tomato. Plant Mol. Biol. 13, 223–232
Prioul, J-L., Reyss, A. (1988) Rapid variation in the content of the RNA of the small subunit of ribulose-1,5-bisphosphate carboxylase of mature tobacco leaves in response to localized changes in light quantity. Relationship between the activity and quantity of the enzyme. Planta 174, 488–494
Ritter, A., Wagner, E., Holmes, M.G. (1981) Light quantity and quality interactions in the control of elongation growth in light-grown Chenopodium rubrum L. seedlings. Planta 153, 556–560
Roscher, E., Zeltsche, K. (1986) The effects of light quality and intensity on the synthesis of ribulose-1,5-bisphosphate carboxylase and its mRNAs in the green alga Chlorogonium elongatum. Planta 167, 582–586
Schwarz, A., Zeiger, E. (1984) Metabolic energy for stomatal opening. Roles of photophosphorylation and oxidative phosphorylation. Planta 161, 129–136
Senger, H., Bauer, B. (1987) The influence of light quality on adaptation and function of the photosynthetic apparatus. Photochem. Photobiol. 45, 936–946
Silverthorne, J., Tobin, E.M. (1987) Phytochrome regulation of nuclear gene expression. Bioessays 7, 18–23
Smith, H. (1982) Light quality, photoperception and plant strategy. Annu. Rev. Plant Physiol. 33, 481–518
Smith, H. (1986) The perception of light quality. In: Photomorphogenesis in plants, pp. 187–217, Kendrick, R.E., Kronenberg, G.H.M., eds. Martinus Nijhoff, Dordrecht
Thompson, W.F., White, M.J. (1991) Physiological and molecular studies of light-regulated nuclear genes in higher plants. Annu. Rev. Plant Physiol. & Plant Mol. Biol. 42, 423–466
Van Volkenburgh, E., Cleland, R.E. (1979) Separation of cell enlargement and division in bean leaves. Planta 146, 245–247
Warpeha, K.M.F., Kaufman, L.S. (1990) Two distinct blue-light responses regulate the levels of transcripts of specific nuclearcoded genes in pea. Planta 182, 553–558
Wehmeyer, B., Cashmore, A.R., Schäfer, E. (1989) Photocontrol of the expression of genes encoding chlorophyll a/b binding proteins and small subunit of ribulose-1,5-bisphosphate carboxylase in etiolated seedlings of Lycopersicon esculentum (L.) and Nicotiana tabacum (L.). Plant Physiol. 93, 990–997
Withers, A.C., Vince-Prue, D., Thomas, B. (1991) Identity of the photoreceptors for the perception of irradiance in photosynthetic light acclimation in tomato. Photochem. Photobiol. 54, 451–457