Standardization of extracellular vesicle measurements by flow cytometry through vesicle diameter approximation

Brisson, 2017, Extracellular vesicles from activated platelets: a semiquantitative cryo‐electron microscopy and immuno‐gold labeling study, Platelets, 28, 1, 10.1080/09537104.2016.1268255 Gasecka, 2017, Platelet extracellular vesicles as biomarkers for arterial thrombosis, Platelets, 28, 228, 10.1080/09537104.2016.1254174 Van Der Pol, 2012, Single vs. swarm detection of microparticles and exosomes by flow cytometry, J Thromb Haemost, 10, 919, 10.1111/j.1538-7836.2012.04683.x van der Pol, 2014, Particle size distribution of exosomes and microvesicles by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing, J Thromb Haemost, 12, 1182, 10.1111/jth.12602 Robert, 2008, Standardization of platelet‐derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?, J Thromb Haemost, 7, 190, 10.1111/j.1538-7836.2008.03200.x Lacroix, 2010, Standardization of platelet‐derived microparticle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop, J Thromb Haemost, 8, 2571, 10.1111/j.1538-7836.2010.04047.x Cointe, 2016, Standardization of microparticle enumeration across different flow cytometry platforms: results of a multicenter collaborative workshop, J Thromb Haemost, 15, 187, 10.1111/jth.13514 van der Vlist, 2012, Fluorescent labeling of nano‐sized vesicles released by cells and subsequent quantitative and qualitative analysis by high‐resolution flow cytometry, Nat Protoc, 7, 1311, 10.1038/nprot.2012.065 Chandler, 2011, A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer, J Thromb Haemost, 9, 1216, 10.1111/j.1538-7836.2011.04283.x Konokhova, 2012, Light‐scattering flow cytometry for identification and characterization of blood microparticles, J Biomed Optics, 17, 057006, 10.1117/1.JBO.17.5.057006 Gardiner, 2014, Measurement of refractive index by nanoparticle tracking analysis reveals heterogeneity in extracellular vesicles, J Extracell Vesicles, 3, 25361, 10.3402/jev.v3.25361 Maetzler, 2002 Bohren, 2007, 82 van der Pol, 2018, Absolute sizing and label‐free identification of extracellular vesicles by flow cytometry, Nanomedicine, 14, 801, 10.1016/j.nano.2017.12.012 van der Pol, 2014, Refractive index determination of nanoparticles in suspension using nanoparticle tracking analysis, Nano Lett, 14, 6195, 10.1021/nl503371p Thompson, 1982, Size dependent platelet subpopulations: relationship of platelet volume to ultrastructure, enzymatic activity, and function, Br J Haematol, 50, 509, 10.1111/j.1365-2141.1982.tb01947.x Davis, 1998, Determination of CD4 antigen density on cells: role of antibody valency, avidity, clones, and conjugation, Cytometry, 33, 197, 10.1002/(SICI)1097-0320(19981001)33:2<197::AID-CYTO14>3.0.CO;2-P Chase, 1998, Resolution of dimly fluorescent particles: a practical measure of fluorescence sensitivity, Cytometry, 33, 267, 10.1002/(SICI)1097-0320(19981001)33:2<267::AID-CYTO24>3.0.CO;2-R Stoner, 2016, High sensitivity flow cytometry of membrane vesicles, Cytometry Part A, 89, 196, 10.1002/cyto.a.22787 de Rond, 2018, Comparison of generic fluorescent dyes for detection of extracellular vesicles by flow cytometry, Clin Chem, 64, 680, 10.1373/clinchem.2017.278978