Frequently asked questions about in vivo chlorophyll fluorescence: practical issues

Photosynthesis Research - Tập 122 - Trang 121-158 - 2014
Hazem M. Kalaji1, Gert Schansker2, Richard J. Ladle3, Vasilij Goltsev4, Karolina Bosa5, Suleyman I. Allakhverdiev6,7, Marian Brestic8, Filippo Bussotti9, Angeles Calatayud10, Piotr Dąbrowski11, Nabil I. Elsheery12, Lorenzo Ferroni13, Lucia Guidi14, Sander W. Hogewoning15, Anjana Jajoo16, Amarendra N. Misra17, Sergio G. Nebauer18, Simonetta Pancaldi13, Consuelo Penella10, DorothyBelle Poli19, Martina Pollastrini9, Zdzislawa B. Romanowska-Duda20, Beata Rutkowska21, João Serôdio22, Kancherla Suresh23, Wiesław Szulc21, Eduardo Tambussi24, Marcos Yanniccari24, Marek Zivcak8
1Department of Plant Physiology, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Warsaw, Poland
2Chêne-Bougeries, Switzerland
3Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, Brazil
4Department of Biophysics and Radiobiology, Faculty of Biology, St. Kliment Ohridski University of Sofia, Sofia, Bulgaria
5Department of Pomology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, SGGW, Warsaw, Poland
6Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
7Institute of Basic Biological Problems, Russian Academy of Sciences, Moscow Region, Russia
8Department of Plant Physiology, Slovak Agricultural University, Nitra, Slovak Republic
9Department of Agri-Food Production and Environmental Science (DISPAA), University of Florence, Florence, Italy
10Departamento de Horticultura, Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain
11Department of Environmental Improvement, Faculty of Civil and Environmental Engineering, Warsaw University of Life Sciences – SGGW, Warsaw, Poland
12Agricultural Botany Department, Faculty of Agriculture, Tanta University, Tanta, Egypt
13Department of Life Sciences and Biotechnologies, University of Ferrara, Ferrara, Italy
14Department of Agriculture, Food and Environment, Pisa, Italy
15Plant Lighting BV, Bunnik, The Netherlands
16School of Life Sciences, Devi Ahilya University, Indore, India
17Centre for Life Sciences, Central University of Jharkhand, Ranchi, India
18Departamento de Producción vegetal, Universitat Politècnica de València, Valencia, Spain
19Department of Biology, Roanoke College, Salem, USA
20Department of Ecophysiology and Plant Development, University of Lodz, Lodz, Poland
21Agricultural Chemistry Department, Faculty of Agriculture and Biology, Warsaw University of Life Sciences – SGGW, Warsaw, Poland
22Departamento de Biologia & CESAM (Centro de Estudos do Ambiente e do Mar), Universidade de Aveiro, Aveiro, Portugal
23Directorate of Oil Palm Research, Pedavegi, India
24Institute of Plant Physiology, INFIVE (Universidad Nacional de La Plata – Consejo Nacional de Investigaciones Científicas y Técnicas), La Plata, Argentina

Tóm tắt

The aim of this educational review is to provide practical information on the hardware, methodology, and the hands on application of chlorophyll (Chl) a fluorescence technology. We present the paper in a question and answer format like frequently asked questions. Although nearly all information on the application of Chl a fluorescence can be found in the literature, it is not always easily accessible. This paper is primarily aimed at scientists who have some experience with the application of Chl a fluorescence but are still in the process of discovering what it all means and how it can be used. Topics discussed are (among other things) the kind of information that can be obtained using different fluorescence techniques, the interpretation of Chl a fluorescence signals, specific applications of these techniques, and practical advice on different subjects, such as on the length of dark adaptation before measurement of the Chl a fluorescence transient. The paper also provides the physiological background for some of the applied procedures. It also serves as a source of reference for experienced scientists.

Tài liệu tham khảo

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