Evaluation of Lymphocyte Response to the Induced Oxidative Stress in a Cohort of Ageing Subjects, including Semisupercentenarians and Their Offspring

Mediators of Inflammation - Tập 2018 - Trang 1-14 - 2018
Federico Sizzano1, Sebastiano Collino2, Ornella Cominetti2, Daniela Monti3, Paolo Garagnani4,5,6,7,8,9, Rita Ostan7, Chiara Pirazzini10, Maria Giulia Bacalini10, Daniela Mari11,12, Giuseppe Passarino13, Claudio Franceschi6,7,9, Alessio Palini1
1Flow Cytometry, Nestlè Institute of Health Sciences, EPFL Campus, 1010 Lausanne, Switzerland
2Molecular Biomarkers, Nestlè Institute of Health Sciences, EPFL Campus, 1010 Lausanne, Switzerland
3Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134, Florence, Italy
4Center for Applied Biomedical Research, St. Orsola-Malpighi University Hospital, 40138 Bologna, Italy
5Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institute at Huddinge University Hospital, S-141 86 Stockholm, Sweden
6Department of Experimental, Diagnostic and Specialty Medicine Experimental Pathology, University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy
7Interdepartmental Centre “L. Galvani” (CIG), University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy
8Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopedic Institute, Bologna
9Unit of Bologna, CNR Institute for Molecular Genetics, Bologna, Italy
10IRCCS, Institute of Neurological Sciences of Bologna, Ospedale Bellaria, Via Altura 3, 40139 Bologna, Italy
11Fondazione Ca' Granda, IRCCS Ospedale Maggiore Policlinico, Via Francesco Sforza 35, 20122 Milan, Italy
12Geriatric Unit, Department of Medical Sciences and Community Health, University of Milan, Via Pace 9, 20122 Milan, Italy
13Department of Ecology, Biology, and Earth Sciences, University of Calabria, Rende, Italy

Tóm tắt

The production of reactive oxygen species (ROS) may promote immunosenescence if not counterbalanced by the antioxidant systems. Cell membranes, proteins, and nucleic acids become the target of ROS and progressively lose their structure and functions. This process could lead to an impairment of the immune response. However, little is known about the capability of the immune cells of elderly individuals to dynamically counteract the oxidative stress. Here, the response of the main lymphocyte subsets to the induced oxidative stress in semisupercentenarians (CENT), their offspring (OFF), elderly controls (CTRL), and young individuals (YO) was analyzed using flow cytometry. The results showed that the ratio of the ROS levels between the induced and noninduced (I/NI) oxidative stress conditions was higher in CTRL and OFF than in CENT and YO, in almost all T, B, and NK subsets. Moreover, the ratio of reduced glutathione levels between I/NI conditions was higher in OFF and CENT compared to the other groups in almost all the subsets. Finally, we observed significant correlations between the response to the induced oxidative stress and the degree of methylation in specific genes on the oxidative stress pathway. Globally, these data suggest that the capability to buffer dynamic changes in the oxidative environment could be a hallmark of longevity in humans.

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Tài liệu tham khảo

10.1016/j.mad.2016.01.001

10.18632/aging.101116

10.1038/nri3547

10.4049/jimmunol.1401174

10.1038/ni.2018

10.1084/jem.20050882

10.1016/j.exger.2016.02.005

10.2174/1381612811319090016

10.1016/b978-0-12-642760-8.50005-3

10.1016/S0140-6736(94)92211-X

10.1155/2012/936486

10.1515/hsz-2014-0312

10.3109/08830185.2012.755176

10.4049/jimmunol.0901662

10.1016/j.mad.2013.02.008

10.1074/jbc.M112.381996

10.1080/08916930701574331

10.2337/dc12-1030

10.1016/j.biocel.2007.01.002

10.1016/j.cell.2014.10.039

10.1016/j.mad.2016.12.008

10.1155/2010/380460

10.1371/journal.pone.0056564

10.1016/S0891-5849(00)00246-X

10.1186/1742-4933-5-13

10.4161/oxim.3.4.12860

10.1371/journal.pone.0091005

10.1016/j.redox.2016.12.026

10.1111/j.1532-5415.2008.02018.x

10.18632/aging.100861

10.18632/aging.100894

10.1186/1742-4933-5-6

10.3389/fmicb.2016.02111

1993, Blood, 82, 2767, 10.1182/blood.V82.9.2767.2767

2000, Blood, 95, 2860, 10.1182/blood.V95.9.2860.009k35_2860_2868

10.1111/j.1474-9726.2006.00204.x

10.1016/s0047-6374(00)00220-7

10.3390/ijms140816443

10.1111/acel.12005

10.1016/j.mad.2017.01.006

10.1007/s11357-012-9463-1

10.1139/Y10-001

10.1016/B978-0-12-420117-0.00003-7

10.1038/srep02704

10.1186/1471-2164-13-636

10.1155/2016/5957404

10.1371/journal.pone.0057002

10.1093/carcin/19.10.1873

10.1016/j.abb.2016.01.017

10.1016/j.mam.2008.05.005

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Mediators of Inflammation

Tập 2018

1-14

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