Common co‐lipids, in synergy, impart high gene transfer properties to transfection‐incompetent cationic lipids

FEBS Letters - Tập 579 - Trang 1291-1300 - 2005
Koushik Mukherjee1, Joyeeta Sen1, Arabinda Chaudhuri1
1Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad 500 007, India

Tóm tắt

Efficacious cationic transfection lipids usually need either DOPE or cholesterol as co‐lipid to deliver DNA inside the cell cytoplasm in non‐viral gene delivery. If both of these co‐lipids fail in imparting gene transfer properties, the cationic lipids are usually considered to be transfection inefficient. Herein, using both the reporter gene assay in CHO, COS‐1 and HepG2 cells and the whole cell histochemical X‐gal staining assay in representative CHO cells, we demonstrate that common co‐lipids DOPE, Cholesterol and DOPC, when act in synergy, are capable of imparting improved gene transfer properties to a novel series of cationic lipids (1–5). Contrastingly, lipids 1–5 became essentially transfection‐incompetent when used in combination with each of the pure co‐lipid components alone.

Tài liệu tham khảo

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FEBS Letters

Tập 579

1291-1300

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