Early Signal Transduction by the Antigen Receptor Without Commitment to T Cell Activation

American Association for the Advancement of Science (AAAS) - Tập 240 Số 4855 - Trang 1029-1031 - 1988
Mark A. Goldsmith1, Arthur Weiss1
1Departments of Medicine and Microbiology and Immunology, University of California, San Francisco, and Howard Hughes Medical Institute, San Francisco, CA 94143.

Tóm tắt

The T lymphocyte antigen-receptor complex mediates antigen-specific cell activation, at least in part, through the production of inositolphospholipid-derived second messengers. Little is known about how second messenger events, typically measured within minutes of ligand binding, eventually lead to distal biologic responses such as expression of lymphokine genes. Several monoclonal antibodies directed against the receptor complex were tested for their ability to elicit transmembrane signaling in the parental Jurkat line and in a somatic mutant (J.CaM1) with a deficient receptor function. One antibody elicited substantial early Ca 2+ mobilization responses in both cells but was unable to promote expression of the interleukin-2 gene in J.CaM1. In J.CaM1 there was a diminished production of phosphatidylinositol second messengers, and the elevation in intracellular free Ca 2+ was transient. Thus, short-term Ca 2+ mobilization does not always indicate complete signal transmission and lead to a full cellular response.

Từ khóa


Tài liệu tham khảo

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American Association for the Advancement of Science (AAAS)

Tập 240 Số 4855

1029-1031

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