Injection of a soluble sperm fraction into sea-urchin eggs triggers the cortical reaction
Tóm tắt
Fertilization membranes from around unfertilized sea-urchin eggs after microinjection of a soluble spermatozoa fraction isosmotic with seawater. This demonstrates that the spermatozoon contains a chemical that triggers an increase in cytosolic calcium, leading to exocytosis of cortical granules. It also demonstrates that the triggering mechanism does not require an externally-activated egg-membrane process. Further experiments show that the chemical trigger is not calcium.
Tài liệu tham khảo
Schuel, H., Gamete Res.1 (1978) 299.
Vacquier V. D., Devl Biol.43 (1975) 62.
Zucker, R. S., and Steinhardt, R. A., Biochim. biophys. Acta541 (1978) 459.
Baker, P. F., Knight, D. E., and Whitaker, M. J., Proc. R. Soc. Lond.B 207 (1980) 149.
Hamaguchi, Y., and Hiramoto, Y., Exp. Cell Res.134 (1981) 171.
Hiramoto, Y., Exp. Cell Res.27 (1962) 416.
Patton, J., and Reeder, W., Analyt. Chem.28 (1956) 1026.
Hollinger, T. G., and Schuetz, A. W., J. Cell Biol.71 (1976) 395.
Epel, D., in: Ions, Cell Proliferation, and Cancer, p. 327. Academic Press, New York 1982.
Poenie, M., Patton, C., and Epel, D., J. Cell Biol.95 (1982) 161a.
Robertson, T. B., Arch. Entwicklungsmech35 (1912) 64.
Loeb, J., in: Artificial Parthenogenesis and Fertilization. University Press Chicago 1913.
Lillie, R. S., and Baskervill, M. L., Am. J. Physiol.61 (1922) 272.
Mazia, D., Schatten, G., and Steinhardt, R., Proc. natn. Acad. Sci. USA72 (1975) 4469.