Dual mode of energy coupling by the oxyanion-translocating ArsB protein

Journal of Bacteriology - Tập 177 Số 2 - Trang 385-389 - 1995
Saikat Dey1, Barry P. Rosen1
1Department of Biochemistry, Wayne State University, School of Medicine, Detroit, Michigan 48201.

Tóm tắt

The arsA and arsB genes of the ars operon of R-factor R773 confer arsenite resistance in Escherichia coli by coding for an anion-translocating ATPase. Arsenite resistance and the in vivo energetics of arsenite transport were compared in cells expressing the arsA and arsB genes and those expressing just the arsB gene. Cells expressing the arsB gene exhibited intermediate arsenite resistance compared with cells expressing both the arsA and arsB genes. Both types of cells exhibited energy-dependent arsenite exclusion. Exclusion of 73AsO2- from cells expressing only the arsB gene was coupled to electrochemical energy, while in cells expressing both genes, transport was coupled to chemical energy, most likely ATP. These results suggest that the Ars anion transport system can be either an obligatory ATP-coupled primary pump or a secondary carrier coupled to the proton motive force, depending on the subunit composition of the transport complex.

Từ khóa


Tài liệu tham khảo

Angov , E. , and W. S. A. Brusilow . 1988 . Use of lac fusions to measure in vivo regulation of expression of Escherichia coli proton-translocating ATPase (unc) genes . J. Bacteriol. 170 : 459 - 462 .

Berger , E. A. 1973 . Different mechanisms for energy coupling for the active transport of proline and glutamine in Escherichia coli . Proc. Natl. Acad. Sci. USA 70 : 1514 - 1518 .

Bolivar , F. , R. L. Rodriguez , P. J. Greene , M. Betlach , H. L. Heyneker , H. Boyer , J. H. Crosa , and S. Falkow . 1977 . Construction and characterization of a new cloning vehicles. II. A multipurpose cloning system . Gene 2 : 95 - 113 .

Bröer , S. , G. Ji , A. Bröer , and S. Silver . 1993 . Arsenic efflux governed by the arsenic resistance determinant of Staphylococcus aureus plasmid pI258 . J. Bacteriol. 175 : 3480 - 3485 .

Chen , C. - M. , T. Misra , S. Silver , and B. P. Rosen . 1986 . Nucleotide sequence of the structural genes for an anion pump: the plasmid-encoded arsenical resistance operon . J. Biol. Chem. 261 : 15030 - 15038 .

Dey , S. , D. Dou , and B. P. Rosen . 1994 . ATP-dependent arsenite transport in everted membrane vesicles of Escherichia coli . J. Biol. Chem. 269 : 25442 - 25446 .

Dey , S. , D. Dou , L. S. Tisa , and B. P. Rosen . 1994 . Interaction of the catalytic and the membrane subunits of an oxyanion-translocating ATPase . Arch. Biochem. Biophys. 311 : 418 - 424 .

Dou D. S. Dey and B. P. Rosen. 1994. A functional chimeric membrane subunit of an ion-translocating ATPase. Antonie van Leeuwenhoek 65:359-368.

Dou , D. , J. B. Owalabi , S. Dey , and B. P. Rosen . 1992 . Construction of a chimeric ArsA-ArsB protein for overexpression of the oxyanion-translocating ATPase . J. Biol. Chem. 267 : 25768 - 25775 .

Hedges , R. W. , and S. Baumberg . 1973 . Resistance to arsenic compounds conferred by a plasmid transmissible between strains of Escherichia coli . J. Bacteriol. 115 : 459 - 460 .

Hsu , C. M. , and B. P. Rosen . 1989 . Characterization of the catalytic subunit of an anion pump . J. Biol. Chem. 264 : 17349 - 17354 .

Ji , G. , and S. Silver . 1992 . Regulation and expression of the arsenic resistance operon from Staphylococcus aureus plasmid pI258 . J. Bacteriol. 174 : 3684 - 3694 .

Kaback , H. R. 1990 . Active transport: membrane vesicles, bioenergetics, molecules and mechanisms . Bacteria 12 : 151 - 202 .

Karkaria , C. E. , C. M. Chen , and B. P. Rosen . 1990 . Mutagenesis of a nucleotide binding site of an anion-translocating ATPase . J. Biol. Chem. 265 : 7832 - 7836 .

Kaur , P. , and B. P. Rosen . 1992 . Plasmid-encoded resistance to arsenic and antimony . Plasmid 27 : 29 - 40 .

Maloney , P. C. 1990 . A consensus structure for membrane transport . Res. Microbiol. 141 : 374 - 383 .

Miller J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

Mobley , H. L. , and B. P. Rosen . 1982 . Energetics of plasmid-mediated arsenate resistance in Escherichia coli . Proc. Natl. Acad. Sci. USA 79 : 6119 - 6122 .

Neufeld , H. A. , R. D. Towner , and J. Pace . 1975 . A rapid method for determining ATP by the firefly Iuciferin-luciferase system . Experientia 31 : 391 - 392 .

Reay , P. F. , and C. J. Asher . 1977 . Preparation and purification of74Aslabeled arsenate and arsenite for use in biological experiments . Anal. Biochem. 78 : 557 - 560 .

Rosen , B. P. 1973 . ~-Galactoside transport and proton movements in an adenosine triphosphatase deficient mutant of Escherichia coli . Biochem. Biophys. Res. Commun. 53 : 1289 - 1296 .

Rosen , B. P. , and M. G. Borbolla . 1984 . A plasmid-encoded arsenite pump produces resistance in Escherichia coli . Biochem. Biophys. Res. Commun. 124 : 760 - 765 .

Rosen , B. P. , W. Weigel , C. E. Karkaria , and P. Gangola . 1988 . Molecular characterization of an anion pump. The arsA gene product is an arsenite(antimonate)-stimulated ATPase . J. Biol. Chem. 263 : 3067 - 3070 .

Rosenstein , R. , P. Peschel , B. Wieland , and F. Götz . 1992 . Expression and regulation of the Staphylococcus xylosus antimonite, arsenite, and arsenate resistance operon . J. Bacteriol. 174 : 3676 - 3683 .

San Francisco , M. J. D. , C. L. Hope , J. B. Owolabi , L. S. Tisa , and B. P. Rosen . 1990 . Identification of the metalloregulatory element of the plasmidencoded arsenical resistance operon . Nucleic Acids Res. 18 : 619 - 624 .

San Francisco , M. J. D. , L. S. Tisa , and B. P. Rosen . 1989 . Identification of the membrane component of the anion pump encoded by the arsenical resistance operon of R-factor R773 . Mol. Microbiol. 3 : 15 - 21 .

Silver , S. , K. Budd , K. M. Leahy , W. V. Shaw , D. Hammond , R. P. Novick , G. R. Willsky , M. H. Malamy , and H. Rosenberg . 1981 . Inducible plasmiddetermined resistance to arsenate, arsenite, and antimony(III) in Escherichia coli and Staphylococcus aureus . J. Bacteriol. 146 : 983 - 996 .

Silver , S. , and D. Keach . 1982 . Energy-dependent arsenate efflux: the mechanism of plasmid mediated resistance . Proc. Natl. Acad. Sci. USA 79 : 6114 - 6118 .

Sone , N. 1990 . Respiratory-driven proton pumps . Bacteria 12 : 1 - 32 .

Tisa , L. S. , and B. P. Rosen . 1991 . Molecular characterization of an anion pump: the ArsB protein is the membrane anchor for the ArsA protein . J. Biol. Chem. 265 : 190 - 194 .

Wu , J. H. , L. Tisa , and B. P. Rosen . 1992 . Membrane topology of the ArsB protein, the membrane component of an anion-translocating ATPase . J. Biol. Chem. 267 : 12570 - 12576 .

Thông tin
Thông tin xuất bản

Journal of Bacteriology

Tập 177 Số 2

385-389

Thông tin tác giả