Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ

American Association for the Advancement of Science (AAAS) - Tập 272 Số 5268 - Trang 1646-1649 - 1996
John Nelson1, Christopher W. Lawrence1, David C. Hinkle1
1J. R. Nelson and C. W. Lawrence, Department of Biophysics, School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, NY 14642, USA.

Tóm tắt

The REV3 and REV7 genes of the yeast Saccharomyces cerevisiae are required for DNA damage-induced mutagenesis. The Rev3 and Rev7 proteins were shown to form a complex with DNA polymerase activity. This polymerase replicated past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally severely inhibits replication, with an efficiency of ∼10 percent. In contrast, bypass replication efficiency with yeast DNA polymerase α was no more than 1 percent. The Rev3-Rev7 complex is the sixth eukaryotic DNA polymerase to be described, and is therefore called DNA polymerase ζ.

Từ khóa


Tài liệu tham khảo

CHAN G.L., CYCLOBUTANE PYRIMIDINE DIMERS AND (6-4) PHOTOPRODUCTS BLOCK POLYMERIZATION BY DNA-POLYMERASE-I, BIOCHEMISTRY 24, 5723 (1985).

FIELDS S, A NOVEL GENETIC SYSTEM TO DETECT PROTEIN PROTEIN INTERACTIONS, NATURE 340, 245 (1989).

FRIEDBERG E.C., DNA REPAIR (1995).

GEITZ R.D., GENE 74, 527 (1988).

HOLLENBERG S.M., IDENTIFICATION OF A NEW FAMILY OF TISSUE-SPECIFIC BASIC HELIX-LOOP-HELIX PROTEINS WITH A 2-HYBRID SYSTEM, MOLECULAR AND CELLULAR BIOLOGY 15, 3813 (1995).

HOVLAND P, GALACTOSE AS A GRATUITOUS INDUCED OF GAL GENE-EXPRESSION IN YEASTS GROWING ON GLUCOSE, GENE 83, 57 (1989).

LARIMER F.W., THE REV1 GENE OF SACCHAROMYCES-CEREVISIAE - ISOLATION, SEQUENCE, AND FUNCTIONAL-ANALYSIS, JOURNAL OF BACTERIOLOGY 171, 230 (1989).

LARSON K.L., INFLUENCE OF TEMPLATE STRANDEDNESS ON INVITRO REPLICATION OF MUTAGEN-DAMAGED DNA, BIOCHEMISTRY 26, 2471 (1987).

MORRISON A, REV3, A SACCHAROMYCES-CEREVISIAE GENE WHOSE FUNCTION IS REQUIRED FOR INDUCED MUTAGENESIS, IS PREDICTED TO ENCODE A NONESSENTIAL DNA-POLYMERASE, JOURNAL OF BACTERIOLOGY 171, 5659 (1989).

NELSON J.R. unpublished data.

ODAY C.L., PCNA-INDUCED DNA-SYNTHESIS PAST CIS-SYN AND TRANS-SYN-I THYMINE DIMERS BY CALF THYMUS DNA-POLYMERASE DELTA INVITRO, NUCLEIC ACIDS RESEARCH 20, 5403 (1992).

PARK H.O., BUD2 ENCODES A GTPASE-ACTIVATING PROTEIN FOR BUD1/RSR1 NECESSARY FOR PROPER BUD-SITE SELECTION IN YEAST, NATURE 365, 269 (1993).

RABKIN S.D., INVITRO BYPASS OF UV-INDUCED LESIONS BY ESCHERICHIA-COLI DNA-POLYMERASE-I - SPECIFICITY OF NUCLEOTIDE INCORPORATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80, 1541 (1983).

RAJAGOPALAN M, ACTIVITY OF THE PURIFIED MUTAGENESIS PROTEINS UMUC, UMUD', AND RECA IN REPLICATIVE BYPASS OF AN ABASIC DNA LESION BY DNA POLYMERASE-III, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 89, 10777 (1992).

SHIMIZU K, PURIFICATION AND CHARACTERIZATION OF A NEW DNA-POLYMERASE FROM BUDDING YEAST SACCHAROMYCES-CEREVISIAE - A PROBABLE HOMOLOG OF MAMMALIAN DNA-POLYMERASE BETA, JOURNAL OF BIOLOGICAL CHEMISTRY 268, 27148 (1993).

TAYLOR J.S., CIS-SYN THYMINE DIMERS ARE NOT ABSOLUTE BLOCKS TO REPLICATION BY DNA-POLYMERASE-I OF ESCHERICHIA-COLI INVITRO, BIOCHEMISTRY 29, 1624 (1990).

TORPEY L.E., CLONING AND SEQUENCE OF REV7, A GENE WHOSE FUNCTION IS REQUIRED FOR DNA DAMAGE-INDUCED MUTAGENESIS IN SACCHAROMYCES-CEREVISIAE, YEAST 10, 1503 (1994).

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 272 Số 5268

1646-1649

Thông tin tác giả