Chlamydospore formation by Paracoccidioides brasiliensis mycelial form

Revista do Instituto de Medicina Tropical de Sao Paulo - Tập 31 Số 3 - Trang 151-157 - 1989
Marcello Franco1, Ayako Sano2, Kota Kera2, Kazuko Nishimura2, Kanji Takeo2, Makoto Miyaji2
1São Paulo State University, Brazil
2Chiba University, Japan

Tóm tắt

To investigate the role of some adverse environmental conditions in chlamy-dospore formation by the mycelial form of P. brasiliensis, we cultured four P. brasiliensis isolates (18, Bt4, 1183, Pb9) at 25°C within solid agar medium either rich or poor in nutrients. Isolates 18 and 1183 were also cultured under anaerobiosis in a nitrogen atmosphere. Isolate 18 produced great number of terminal and intercalary chlamydospore after 7-10 days of culture in a medium poor in nutrients (2% agar with 0.1% dextrose and polypepton). The three other isolates also produced chlamydospores under the same conditions, but in lower numbers. Chlamydospore production by isolate 18 was abolished when the fungus was cultured in two agar media rich in nutrients (brain heart infusion and potato dextrose agar). Anaerobic incubation of isolate 18 under an atmosphere of N2 showed small mycelial outgrowth with numerous chlamydospores. At the electron microscopical level, the chlamydospores showed one or various nuclei and numerous mitochondria, indicating great potential for further development. Accordingly, chlamydospores produced multiple budding after only 24 h incubation at 35°C. The results demonstrate that under adverse environmental conditions P. brasiliensis mycelial form produces chlamydospores within a short period of time.

Từ khóa


Tài liệu tham khảo

BARTNICKI-GARCIA S., 1961, Thiamine and nicotinic acid: anaerobic growth factors for Mucor rouxii, J. Bact., 82, 142, 10.1128/JB.82.1.142-148.1961

BARTNICKI-GARCIA S., 1962, Nutrition, growth, and morphogenesis of Mucor rouxii, J. Bact., 84, 841, 10.1128/JB.84.4.841-858.1962

BUSTAMANTE B., 1985, Characteristics of the conidia produced by the mycelial form of Paracoccidioides brasiliensis, J. med. vet. Mycol., 23, 407, 10.1080/00362178585380601

CARBONELL L. M., 1965, Transformation of mycelial and yeast forms of Paracoccidioides brasiliensis in cultures and in experimental inoculations, J. Bact., 90, 504, 10.1128/JB.90.2.504-510.1965

CARBONELL L. M., 1968, Mycelial phase of Paracoccidioides brasiliensis and Blastomyces der matitidis: an electron microscope study, J. Bact., 96, 533, 10.1128/JB.96.2.533-543.1968

CARMICHAEL J. W., 1971, Blastospores, aleuriospores, ehlamydospores, 50

CONANT N. F., 1942, The similarity of the fungi causing South American blastomycosis (paracoccidioidal granuloma) and North American blastomycosis (Gilchrist's disease), J. invest. Derm., 5, 353, 10.1038/jid.1942.50

FRANCO M., 1987, Paracoccidioidomycosis: a recently proposed classification of its clinical forms, Rev. Soc. bras. Med. trop., 20, 129, 10.1590/S0037-86821987000200012

GRIFFITHS D. A., 1974, The origin, structure and function of chlamydospore in fungi, Nova Hedwigia, 25, 503

HAWKSWORTH D. L., 1983

JANSONS V. K., 1970, Induction, morphogenesis, and germination of the chlamydospores of Candida albicans, J. Bact., 104, 910, 10.1128/JB.104.2.910-921.1970

LIU P., 1955, Rapid chlamydospore formation by Candida albicans in a buffered alkaline medium, Amer. J. clin. Path., 25, 93 97

McEWEN J. G., 1987, Experimental murine paracoccidioidomycosis induced by the inhalation of conidia, J. med. vet. Mycol., 25, 165, 10.1080/02681218780000231

McVEIGH I., 1972, Factors affecting mycelial to yeast phase conversion and growth of the yeast phase of Histoplasma capsulatum, Mycopathologia (Den Haag), 47, 135

MILLER S. E., 1974, Electron microscopy of young Candida albicans chlamydospores, J. Bact., 119, 992, 10.1128/JB.119.3.992-999.1974

MIYAJI M., 1987, Experimental fungal infections, 1

NEVES J. A., 1951, Researches on the etiological agents of the American blastomycosis: I. Morphology and systematic of the Lutz's disease agent, Mycopathologia (Den Haag), 5, 133

POLLAK L., 1971, Aleuriospores of Paracoccidioides brasiliensis, Mycopathologia (Den Haag), 45, 217, 10.1007/BF02051968

RESTREPO A., 1970, A reappraisal of the microscopical appearance of the mycelial phase of Paracoccidioides brasiliensis, Sabouraudia, 8, 141 144

RESTREPO A., 1985, The ecology of Paracoccidioides brasiliensis: a puzzle still unsolved, J. med. vet. Mycol., 23, 323, 10.1080/00362178585380481

RESTREPO A., 1981, Recovery of Paracoccidioides brasiliensis from a partially calcified lymph node lesion by mycroaerophilic incubation of liquid medium, Sabouraudia, 19, 295, 10.1080/00362178185380471

RESTREPO A., 1986, A technique to collect and dislodge conidia produced by Paracoccidioides brasiliensis mycelial form, J. med. vet. Mycol., 24, 247 250

RESTREPO B. I., 1986, Morphological development of the conidia produced by Paracoccidioides brasiliensis mycelial form, J. med. vet. Mycol., 24, 337 339

SANBLAS F., 1986, Ultrastructure of spore formation in Paracoccidioides brasiliensis, J. med. vet. Mycol., 24, 203, 10.1080/02681218680000301

SZANISZLO P. J., 1983, Dimorphism: morphological and biochemical aspects, 323

TAKEO K., 1974, Ultrastructure of polymorphic Mucor as observed by means of freeze-etching: III. Dormant sporangiospore, Arch. Microbiol., 99, 99, 10.1007/BF00696226

Thông tin
Thông tin xuất bản

Revista do Instituto de Medicina Tropical de Sao Paulo

Tập 31 Số 3

151-157

Thông tin tác giả