Characterization and evolution of two bacteriome‐inhabiting symbionts in cixiid planthoppers (Hemiptera: Fulgoromorpha: Pentastirini)

Wiley - Tập 11 Số 12 - Trang 3265-3279 - 2009
Alberto Bressan1,2, Joel D. Arneodo1,3, Mauro Simonato4, William P. Haines2, E. Boudon‐Padieu1
1Biologie et Écologie des Phytoplasmes, UMR PME INRA – CNRS – Université de Bourgogne, BP 86510-21065 DIJON Cedex, France.
2Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA
3Present address: CONICET, IMYZA-INTA, C. C. 25 (1712) Castelar, Buenos Aires, Argentina.
4Università di Padova, Dipartimento di Agronomia Ambientale e Produzioni Vegetali, Agripolis Viale dell' Università16 a, 35020 Legnaro PD, Italy.

Tóm tắt

SummaryLike other plant sap‐sucking insects, planthoppers within the family Cixiidae (Insecta: Hemiptera: Fulgoromorpha) host a diversified microbiota. We report the identification and first molecular characterization of symbiotic bacteria in cixiid planthoppers (tribe: Pentastirini). Using universal eubacterial primers we first screened the eubacterial 16S rRNA sequences in Pentastiridius leporinus (Linnaeus) with PCR amplification, cloning, and restriction fragment analysis. We identified three main 16S rRNA sequences that corresponded to a Wolbachia bacterium, a plant pathogenic bacterium, and a novel gammaproteobacterial symbiont. A fourth bacterial species affiliated with ‘Candidatus Sulcia muelleri’ was detected in PCR assays using primers specific for the Bacteroidetes. Within females of two selected cixiid planthoppers, P. leporinus and Oliarus filicicola, fluorescence In situ hybridization analysis and transmission electron microscopy observations showed that ‘Ca. Sulcia muelleri’ and the novel gammaproteobacterial symbiont were housed in separate bacteriomes. Phylogenetic analysis revealed that both of these symbionts occurred in at least four insect genera within the tribe Pentastirini. ‘Candidatus Purcelliella pentastirinorum’ was proposed as the novel gammaproteobacterial symbiont.

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Wiley

Tập 11 Số 12

3265-3279

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