Photo CIDNP study on the complex formation of λ‐ cro protein with OR3

FEBS Letters - Tập 181 Số 2 - Trang 286-290 - 1985
Masahiro Shirakawa1, Sang Jeon Lee1, Hideo Akutsu1, Yoshimasa Kyōgoku1, Katsuhiko Kitano2, M. Shin2, Eiko Ohtsuka2, Morio Ikehara2
1Institute for Protein Research, Osaka Univ., Suita 565 Japan
2Faculty of Pharmaceutical Sciences, Osaka University, Suita 565, Japan

Tóm tắt

A photo CIDNP spectrum of λ cro repressor protein showed that one of the three tyrosines and His 35 are quite accessible to the photosensitive dye. For the remaining two tyrosine residues one is slightly accessible, but the other is inaccessible. In comparison with the result of differential nitration at tyrosine side chains followed by the peptide analysis, it can be concluded that Tyr 26 is mostly exposed and Tyr 51 is slightly exposed on the surface of the cro dimer. On the addition of OR3 17mer, His 35 and Tyr 26 are no longer accessible to the dye, which indicates that they are involved in interaction. However, a similar phenomenon was observed by adding CAP binding site 22mer. The interaction mechanism will be discussed.

Từ khóa


Tài liệu tham khảo

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Thông tin xuất bản

FEBS Letters

Tập 181 Số 2

286-290

Thông tin tác giả