Submicrometer Intracellular Chemical Optical Fiber Sensors

American Association for the Advancement of Science (AAAS) - Tập 258 Số 5083 - Trang 778-781 - 1992
Weihong Tan1, Zhong-You Shi1, Steven J. Smith1, Duane Birnbaum1, Raoul Kopelman1
1§§Department of Chemistry, University of Michigan, Ann Arbor, MI 48109

Tóm tắt

A thousandfold miniaturization of immobilized optical fiber sensors, a millionfold or more sample reduction, and at least a hundredfold shorter response time, all simultaneously, were achieved by combining nanofabricated optical fiber tips with near-field photopolymerization. Specifically, pH optical fiber sensors were prepared with internal calibration, making use of the differences in both fluorescence and absorption of the acidic and basic dye species. The submicrometer sensors have excellent detection limits, as well as photostability, reversibility, and millisecond response times. Successful applications include intracellular and intraembryonic measurements. Potential applications include spatially and temporally resolved chemical analysis and kinetics inside single biological cells and their substructures.

Từ khóa


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American Association for the Advancement of Science (AAAS)

Tập 258 Số 5083

778-781

Thông tin tác giả