Deletion mapping of chromosome II in carcinoma of the bladder

Genes Chromosomes and Cancer - Tập 13 Số 1 - Trang 1-8 - 1995
Margaret E. Shaw1, Margaret A. Knowles1
1Molecular Genetics Laboratory, Marie Curie Research Institute, Oxted, Surrey, United Kingdom

Tóm tắt

AbstractDeletions of the short arm of chromosome II have been identified by both cytogenetic and molecular criteria in bladder and other types of solid tumor, indicating the presence of one or more suppressor loci in this region. To localize the II p deletion target(s) more precisely and to screen for loss of heterozygosity (LOH) on the long arm of the chromosome, I00 bladder tumors were analyzed for LOH on chromosome II using restriction fragment length polymorphism (RFLPs) and microsatellite markers mapped to both II p and II q. Thirty‐four tumors were found to have LOH at I or more loci. Of these, I7 had LOH restricted to II p, I3 had LOH of both II p and II q, and 4 had LOH of II q only. Eight tumors showed LOH at all informative loci indicating probable loss of an entire copy of chromosome II. A common region of deletion was defined on II p between DIIS922(IIpI5.5) and DlIS569 (IIpI5.I‐I5.2). This region does not include the HRAS or WTI loci (at IIpI5.5and IIp13, respectively). Seventeen tumors had LOH on II q, 4 of which had LOH on II q only. The common region of deletion on II q was between FGF3 and DI IS490 (IIq I3‐q23.2). Two tumors showed LOH on both II p and II q with a clear region of retention of heterozygosity between, indicatinn the existence of two deletion targets on chromosome II. © 1995 Wiley‐Liss, Inc.

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Genes Chromosomes and Cancer

Tập 13 Số 1

1-8

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