Masae Iino1,2, Kaori Goto1,2, Wataru Kakegawa1,2, Haruo Okado1,3, Makoto Sudo2, Shogo Ishiuchi1,2, Akiko Miwa1,3, Yukihiro Takayasu1,2, Izumu Saito4, Keisuke Tsuzuki1,2, Seiji Ozawa1,2
1Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan
2Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan.
3Department of Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Fuchu 183-8526, Japan.
4Institute of Medical Science, University of Tokyo, Tokyo 108-0071, Japan.
Tóm tắt
Glial cells express a variety of neurotransmitter receptors. Notably, Bergmann glial cells in the cerebellum have Ca
2+
-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)–type glutamate receptors (AMPARs) assembled without the GluR2 subunit. To elucidate the role of these Ca
2+
-permeable AMPARs, we converted them into Ca
2+
-impermeable receptors by adenoviral-mediated delivery of the GluR2 gene. This conversion retracted the glial processes ensheathing synapses on Purkinje cell dendritic spines and retarded the removal of synaptically released glutamate. Furthermore, it caused multiple innervation of Purkinje cells by the climbing fibers. Thus, the glial Ca
2+
-permeable AMPARs are indispensable for proper structural and functional relations between Bergmann glia and glutamatergic synapses.
