Functional Human Corneal Equivalents Constructed from Cell Lines
Tóm tắt
Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological, biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked human corneas in key physical and physiological functions, including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.
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Tài liệu tham khảo
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Supported by grants from the Natural Sciences and Engineering Research Council of Canada Medical Research Council–Pharmaceutical Manufacturers' Association of Canada and the P&G International Program for Animal Alternatives. We thank S. Whelan and P. Berg for Ad5 and psv3neo plasmids C. Smith and Ottawa Hospital Research Institute colleagues and E. Hay and A. Tashjian Jr. for helpful insights during preparation of this manuscript.