Multilocus sequence typing suggests the chytrid pathogen of amphibians is a recently emerged clone

Molecular Ecology - Tập 12 Số 2 - Trang 395-403 - 2003
Erica A. Morehouse1, Timothy Y. James1, Austen R. D. Ganley1, Rytas Vilgalys1, Lee Berger2,3, Peter Murphy4, Joyce E. Longcore5
1Department of Biology, Biological Sciences Building, Room 139, Duke University, Durham, NC 27708, USA,
2Australian Animal Health Laboratory, Commonwealth Scientific Industrial Research Organization, Geelong 3200, Victoria, Australia,
3School of Public Health and Tropical Medicine, James Cook University, Townsville 4811, Queensland, Australia,
4Department of Zoology, Southern Illinois University, Carbondale, IL 62901, USA
5Department Biological Sciences, University of Maine, Orono, ME 04469, USA

Tóm tắt

AbstractChytridiomycosis is a recently identified fungal disease associated with global population declines of frogs. Although the fungus, Batrachochytrium dendrobatidis, is considered an emerging pathogen, little is known about its population genetics, including the origin of the current epidemic and how this relates to the dispersal ability of the fungus. In this study, we use multilocus sequence typing to examine genetic diversity and relationships among 35 fungal strains from North America, Africa and Australia. Only five variable nucleotide positions were detected among 10 loci (5918 bp). This low level of genetic variation is consistent with the description of B. dendrobatidis as a recently emerged disease agent. Fixed (i.e. 100%) or nearly fixed frequencies of heterozygous genotypes at two loci suggested that B. dendrobatidis is diploid and primarily reproduces clonally. In contrast to the lack of nucleotide polymorphism, electrophoretic karyotyping of multiple strains demonstrated a number of chromosome length polymorphisms.

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Tài liệu tham khảo

10.1073/pnas.96.24.14043

Ausubel FM, 1998, Current Protocols in Molecular Biology

Barr DJS, 2001, Chytridiomycota, 93

10.1073/pnas.95.15.9031

Berger L, 1999, Declines and Disappearances of Australian Frogs, 23

10.1093/genetics/144.1.427

10.1046/j.1461-0248.2002.00352.x

10.1016/S0006-3207(00)00132-4

10.7589/0090-3558-38.1.206

10.1073/pnas.93.2.770

10.1016/S0145-305X(99)00028-2

10.1046/j.1365-294x.2000.01020.x

10.3201/eid0506.990601

10.1126/science.287.5452.443

10.1006/fgbi.1999.1164

10.1073/pnas.95.1.388

Gräser Y, 1999, Molecular markers reveal exclusively clonal reproduction in Trichophyton rubrum, Journal of Clinical Microbiology, 37, 3713, 10.1128/JCM.37.11.3713-3717.1999

Iadonato SP, 1996, Methods in Molecular Biology V. 54: YAC Protocols, 75

10.1099/00221287-136-12-2433

10.1046/j.0032-0862.2001.00610.x-i1

10.1046/j.1523-1739.1999.97185.x

10.2307/3761366

10.7589/0090-3558-37.1.1

10.1073/pnas.130193297

10.1177/104063879901100219

RozenS SkaletskyHJ(1997) Primer3. Code available athttp://www‐genome.wi.mit.edu/genome_software/other/primer3.html.

10.5962/bhl.title.5685

Speare R, 2001, Developing Management Strategies to Control Amphibian Diseases: Decreasing the Risks Due to Communicable Diseases, 186

10.1073/pnas.94.18.9869

10.1073/pnas.87.7.2414

10.1128/jb.172.8.4238-4246.1990

10.1023/A:1017007131386

10.1046/j.1365-294x.2000.01021.x

10.1128/mr.59.4.686-698.1995

10.1128/MCB.6.1.195

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Molecular Ecology

Tập 12 Số 2

395-403

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