Protein detection by optical shift of a resonant microcavity

Applied Physics Letters - Tập 80 Số 21 - Trang 4057-4059 - 2002
Frank Vollmer1, Dieter Braun1, Albert Libchaber1, M. Khoshsima2, Iwao Teraoka2, Stephen Arnold2
1Center for Studies in Physics and Biology, Rockefeller University, New York, New York 10021
2Microparticle Photophysics Lab (MP3 L), Polytechnic University, Brooklyn, New York 11201

Tóm tắt

We present an optical biosensor with unprecedented sensitivity for detection of unlabeled molecules. Our device uses optical resonances in a dielectric microparticle (whispering gallery modes) as the physical transducing mechanism. The resonances are excited by evanescent coupling to an eroded optical fiber and detected as dips in the light intensity transmitted through the fiber at different wavelengths. Binding of proteins on the microparticle surface is measured from a shift in resonance wavelength. We demonstrate the sensitivity of our device by measuring adsorption of bovine serum albumin and we show its use as a biosensor by detecting streptavidin binding to biotin.

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Tài liệu tham khảo

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Thông tin
Thông tin xuất bản

Applied Physics Letters

Tập 80 Số 21

4057-4059

Thông tin tác giả