Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation

FEBS Letters - Tập 388 - Trang 238-241 - 1996
Yuri N. Korystov1, Oksana R. Dobrovinskaya1, Vera V. Shaposhnikova1, Lazar Kh. Eidus1
1Laboratory of Cell Radiobiology, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142292, Russian Federation

Tóm tắt

The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [3H]arachidonic acid‐labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis.

Tài liệu tham khảo

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FEBS Letters

Tập 388

238-241

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