Phylogenomic characterization and signs of microevolution in the 2022 multi-country outbreak of monkeypox virus

Nature Medicine - Tập 28 Số 8 - Trang 1569-1572 - 2022
Joana Isidro1, Vítor Borges1, Miguel Pinto1, Daniel Sobral1, João Santos1, Alexandra Nunes1, Verónica Mixão1, Rita Ferreira1, Daniela Santos2, Sílvia Duarte2, Luı́s Vieira2, Maria José Borrego3, Maria Sofia Núncio4, Isabel Lopes de Carvalho4, Ana Pelerito4, Rita Cordeiro4, João Paulo Gomes5
1Genomics and Bioinformatics Unit, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), Lisbon, Portugal
2Technology and Innovation Unit, Department of Human Genetics, National Institute of Health Doutor Ricardo Jorge (INSA), Lisbon, Portugal
3National Reference Laboratory of Sexually Transmitted Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), Lisbon, Portugal
4Emergency Response and Biopreparedness Unit, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), Lisbon, Portugal
5Faculty of Veterinary Medicine, Lusófona University, Lisbon, Portugal

Tóm tắt

AbstractThe largest monkeypox virus (MPXV) outbreak described so far in non-endemic countries was identified in May 2022 (refs. 1–6). In this study, shotgun metagenomics allowed the rapid reconstruction and phylogenomic characterization of the first MPXV outbreak genome sequences, showing that this MPXV belongs to clade 3 and that the outbreak most likely has a single origin. Although 2022 MPXV (lineage B.1) clustered with 2018–2019 cases linked to an endemic country, it segregates in a divergent phylogenetic branch, likely reflecting continuous accelerated evolution. An in-depth mutational analysis suggests the action of host APOBEC3 in viral evolution as well as signs of potential MPXV human adaptation in ongoing microevolution. Our findings also indicate that genome sequencing may provide resolution to track the spread and transmission of this presumably slow-evolving double-stranded DNA virus.

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Nature Medicine

Tập 28 Số 8

1569-1572

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