Effects of Fluoride on Structure and Function of Canine Gastric Mucosa

Digestive Diseases and Sciences - 1997
Gary M. Whitford, David H. Pashley, Robert H. Garman

Tóm tắt

These studies were done to determine the effectsof fluoride (F) on the structure and function of thecanine gastric mucosa and the possible protectiveeffects of 16,16-dimethylprostaglandin E2(dmPGE2). A portion of the stomach with its vascularsupply intact was mounted in a two-compartment chamber,one side of which contained a control solution. Minoreffects were caused by exposure to 1 mmol/liter F. Both 5 and 10 mmol/liter F caused markedincreases in the fluxes of water and Na, K, and H ions;mucus secretion; and tissue swelling and redness. Theextent of these changes did not increase appreciably upon exposure to 50 or 100 mmol/liter F.Histological findings included marked thinning of thesurface cell layer, reduced uptake of PAS stain,localized exfoliation and necrosis of surface cells,acute gastritis, and edema. It was concluded that:(1) the threshold F concentration for effects on thestructure and function of the gastric mucosa wasapproximately 1 mmol/liter; (2) the maximum ornear-maximum effects were caused by 10 mmol/liter F; (3) theeffects persisted for at least 6 hr after the exposure;and (4) dmPGE2 (0.5 μg/ml) did notattenuate the effects induced by F.

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Tài liệu tham khảo

Beal JF, Rock WP: Fluoride gels, a laboratory and clinical study. Br Dent J 140:307-310, 1976

Duxbury AJ, Leach FN, Duxbury JT: Acute fluoride toxicity. Br Dent J 153:64-66, 1982

Riggs BL, Seeman E, Hodgson SF, Taves DR, O'Fallon WM: Effect of the fluoride/calcium regimen on vertebral fracture occurrence in postmenopausal osteoporosis. N Engl J Med 306:446-450, 1982

Spak C-J, Sjöstedt S, Eleborg L, Veress B, Perbeck L, Ekstrand J: Tissue response of gastric mucosa after ingestion of fluoride. Br Med J 298:1686-1687, 1989

Bowie JY, Darlow G, Murray MM: The effect of sodium fluoride on gastric acid secretion. J Physiol 122:203-208, 1953

Bond AM, Hunt JN: The effect of sodium fluoride on the output of some electrolytes from the gastric mucosa of cats. J Physiol 133:317-329, 1956

Reed JD, Smy JR: The effects of sodium fluoride on gastric acid and electrolyte output in the anesthetized cat. J Physiol 301:39-48, 1980

Patterson WB, Stetten de W Jr: A study of gastric HC1 formation. Science 109:256-258, 1949

Assem ESK, Wan BYC: Stimulation of H+ ion secretion from the isolated mouse stomach by sodium fluoride. Experientia 38:369-370, 1982

Easmann RP, Steflik DE, Pashley DH, McKinney RV, Whitford GM: Surface changes in rat gastric mucosa induced by sodium fluoride: A scanning electron microscopic study. J Oral Pathol 13:255-264, 1984

Pashley DH, Allison NB, Easmann RP, McKinney RV, Horner JA, Whitford GM: The effects of fluoride on the gastric mucosa of the rat. J Oral Pathol 13:535-545, 1984

Easmann RP, Pashley DH, Birdsong NL, McKinney RV, Whitford GM: Recovery of rat gastric mucosa following single fluoride dosing. J Oral Pathol 14:779-792, 1985

Lincir I, Stipetic S: The influence of amine fluorides on rat gastric mucosa. Jugosl Physiol Pharmacol Acta 24:121-126, 1988

Moody FG, Durbin RP: Effects of glycine and other instillates on concentration of gastric acid. Am J Physiol 209:122-126, 1965

Miller TA, Kraemer BB, Henagan JM, Foucar CE: Topical 16,16-dimethyl-prostaglandin E2. Effects on gastric morphology, hydrogenion loss and bicarbonate secretion. Dig Dis Sci 28:641-648, 1983

Whitford GM: The Metabolism and Toxicity of Fluoride. Monographs in Oral Science, Vol 13. HM Myers (ed). Basel, Karger, 1989

Henagan JM, Schmidt KL, Miller TA: Prostaglandin prevents aspirin injury in the canine stomach under in vivo but not in vitro conditions. Gastroenterology 97:649-659, 1989

Gutknecht J, Walter A: Hydrofluoric and nitric acid transport through lipid bilayer membranes. Biochim Biophys Acta 644:153-156, 1981

Whitford GM, Schuster GS, Pashley DH, Venkateswarlu P: Fluoride uptake by Streptococcus mutans 6715. Infect Immun 18:680-687, 1977

Whitford GM, Pashley DH, Reynolds KE: Fluoride absorption from the rat urinary bladder: A pH-dependent event. Am J Physiol 232:F10-F15, 1977

Whitford GM, Pashley DH, Stringer GI: Fluoride renal clearance: A pH-dependent event. Am J Physiol 230:527-532, 1976

Whitford GM, Pashley DH: Fluoride absorption: the influence of gastric acidity. Calcif Tissue Int 36:302-307, 1984

Schmidt KL, Henagan JM, Smith GS, Hilburn PJ, Miller TA: Prostaglandin cytoprotection against ethanol-induced gastric injury in the rat. Gastroenterology 88:649-659, 1985

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Digestive Diseases and Sciences

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