Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres

Microbiology (United Kingdom) - Tập 157 Số 5 - Trang 1385-1392 - 2011
M Fallani1, Sergio Amarri2, Agneta Uusijärvi3, Rony A. Adam4, Sheila Khanna5, Margarita Aguilera6,7, Ángel Gil7, José María Vieites7, Elisabeth Norin3, David Young8, Jane Scott9, Joël Doré1, Christine A. Edwards5
1Unité de recherche d'Écologie et Physiologie du Système Digestif
2Dept Paediat
3Karolinska Institutet, Stockholm
4Dept Paediat & Adolescent Med
5University of Glasgow
6 Institut National de la Recherche Agronomique,
7Universidad de Granada = University of Granada
8University of Strathclyde, Glasgow
9Flinders University

Tóm tắt

Although it is well established that early infant feeding has a major influence on the establishment of the gut microbiota, very little is understood about how the introduction of first solid food influences the colonization process. This study aimed to determine the impact of weaning on the faecal microbiota composition of infants from five European countries (Sweden, Scotland, Germany, Italy and Spain) which have different lifestyle characteristics and infant feeding practices. Faecal samples were collected from 605 infants approximately 4 weeks after the introduction of first solid foods and the results were compared with the same infants before weaning (6 weeks of age) to investigate the association with determining factors such as geographical origin, mode of delivery, previous feeding method and age of weaning. Samples were analysed by fluorescencein situhybridization and flow cytometry using a panel of 10 rRNA targeted group- and species-specific oligonucleotide probes. The genusBifidobacterium(36.5 % average proportion of total detectable bacteria),Clostridium coccoidesgroup (14 %) andBacteroides(13.6 %) were predominant after weaning. Similar to pre-weaning, northern European countries were associated with a higher proportion of bifidobacteria in the infant gut microbiota while higher levels ofBacteroidesand lactobacilli characterized southern European countries. As before weaning, the initial feeding method influenced theClostridium leptumgroup andClostridium difficile+Clostridium perfringensspecies, and bifidobacteria still dominated the faeces of initially breast-fed infants. Formula-fed babies presented significantly higher proportions ofBacteroidesand theC. coccoidesgroup. The mode of birth influenced changes in the proportions of bacteroides and atopobium. Although there were significant differences in the mean weaning age between countries, this was not related to the populations of bifidobacteria or bacteroides. Thus, although the faecal microbiota of infants after first complementary foods was different to that before weaning commenced, many of the initial influences on microbiota composition were still evident.

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Thông tin xuất bản

Microbiology (United Kingdom)

Tập 157 Số 5

1385-1392

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