Appearance of Water Channels in Xenopus Oocytes Expressing Red Cell CHIP28 Protein

American Association for the Advancement of Science (AAAS) - Tập 256 Số 5055 - Trang 385-387 - 1992
Gregory M. Preston1, Tiziana Piazza Carroll2, William B. Guggino2, Peter Agre1
1Departments of Medicine and Cell Biology/Anatomy, Johns Hopkins University School of Medicine, Baltimore, MD 21205
2Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205

Tóm tắt

Water rapidly crosses the plasma membrane of red blood cells (RBCs) and renal tubules through specialized channels. Although selective for water, the molecular structure of these channels is unknown. The CHIP28 protein is an abundant integral membrane protein in mammalian RBCs and renal proximal tubules and belongs to a family of membrane proteins with unknown functions. Oocytes from Xenopus laevis microinjected with in vitro-transcribed CHIP28 RNA exhibited increased osmotic water permeability; this was reversibly inhibited by mercuric chloride, a known inhibitor of water channels. Therefore it is likely that CHIP28 is a functional unit of membrane water channels.

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American Association for the Advancement of Science (AAAS)

Tập 256 Số 5055

385-387

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