A Rapid Procedure for the Simultaneous Estimation of the Anticonvulsant Drugs, Ethosuximide, Phenobarbitone, Phenytoin, and Carbamazepine in Serum Using High-Pressure Liquid Chromatography

Annals of Clinical Biochemistry - Tập 20 Số 1 - Trang 37-40 - 1983
D C Turnell1, Susan C Trevor1, Jonathan D. Cooper1
1Department of Biochemistry, Coventry and Warwickshire Hospital, Stoney Stanton Road, Coventry CV1 4FH

Tóm tắt

A rapid isocratic high-pressure liquid chromatography procedure for the analysis of ethosuximide, phenobarbitone, phenytoin, and carbamazepine in serum is described. The method employs a single extraction from serum; alumina treatment of this extract eliminates interference from fast-eluting compounds, allowing rapid chromatography. A linear response is obtained for drug concentrations up to three times the upper limit of the therapeutic ranges. Within-batch and between-batch precisions for the means of these ranges varied from 2·2 to 5·5% and from 3·1 to 7·5%, respectively.

Từ khóa


Tài liệu tham khảo

10.1093/clinchem/22.6.856

10.1093/clinchem/23.7.1284

10.1093/clinchem/25.5.810a

10.1016/S0378-4347(00)81348-1

10.1093/clinchem/22.1.25

In Pippenger CE, Pendry JK, Kutt H (Editors), Antiepileptic Drugs: Quantitative Analysis and Interpretation. Raven press, New York, 1978, Ch 22, p. 253; Ch 23 p 261; Ch 25 p 283; Ch 26 p 297.

Supelco Bulletin 768, Separation of anticonvulsant drugs.

Dijkuis IC, 1979, Pharmm Weekblad, 1, 151, 10.1007/BF02293169

10.1039/jr9600001268

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Thông tin xuất bản

Annals of Clinical Biochemistry

Tập 20 Số 1

37-40

Thông tin tác giả