Sensing of Mycotoxin Producing Fungi in the Processing of Grains
Tóm tắt
Due to the growing demand for rapid, sensitive and inexpensive detection of contaminations in food and feed, both in the field and after harvest, non-invasive and non-destructive spectroscopic methods are reviewed for the direct identification of fungi and mycotoxins on grain and in grain products. Different model samples containing defined contaminations of fungi and/or mycotoxins as well as real-world samples were investigated using spectroscopic methods (steady-state and time-resolved fluorescence spectroscopy, ultraviolet/visible/near infrared (NIR) spectroscopy, two-photon-induced fluorescence (TPIF)). To evaluate the capability of TPIF for on-line and in-line mycotoxin analysis in real-world samples, fundamental parameters of different mycotoxins, such as two-photon cross-sections, were determined in beer and wine. A comparison showed that the disturbance due to background emission originating from matrix constituents is significantly reduced under two-photon excitation conditions, and TPIF can be applied for a qualitative and quantitative analysis of mycotoxins in wine and beer. The combination of data obtained from different spectroscopic methods (such as optimised excitation and emission wavelengths, fluorescence decay times and fluorescence quantum efficiency on the one hand side and NIR spectroscopy on the other side) is promising for the qualitative as well as quantitative identification of fungi and mycotoxins. Moreover, NIR reflection spectra yield additional information on ingredients, moisture content and the presence (or absence) of fungi in the sample. The spectroscopic techniques are complemented by chemometric tools to extract the desired chemical information, e.g. with respect to the presence of contaminations.
Tài liệu tham khảo
Biselli, S. (2006). Analytische Methoden für die Kontrolle von Lebens- und Futtermitteln auf Mykotoxine. Journal für Verbraucherschutz und Lebensmittelsicherheit, 1, 106–114.
Böttcher M (2009) Photophysikalische Eigenschaften von Aflatoxin B1 in verschiedenen Lösungsmitteln und Nachweis des Mykotoxins in Weisswein. Bachelor Thesis, Department of Chemistry, University of Potsdam, Germany.
Chelkowski, J. (1974). Spectral behaviour of aflatoxins in different solvents. Photochemistry and Photobiology, 20, 279–280.
Karoui, R., Cartaud, G., & Dufour, E. (2005). Front-face fluorescence spectroscopy as a rapid and nondestructive tool for differentiating various cereal products: A preliminary investigation. Journal of Agricultural and Food Chemistry, 54, 2027–2034.
Kessler, R. W. (Ed.). (2006). Prozessanalytik. Wiley VCH, Weinheim: Strategien und Fallbeispiele aus der industriellen Praxis.
Kostov, Y., & Rao, G. (2000). Low-Cost optical instrumentation for biomedical measurements. The Review of Scientific Instruments, 71(12), 4361–4374.
Krska, R., & Molinelli, A. (2007). Mycotoxin analysis: state-of-the-art and future trends. Analytical and Bioanalytical Chemistry, 387, 145–148.
Kubelka, P., & Munk, F. (1931). Ein beitrag zur optik der farbanstriche. Zeitschrift für Technische Chemie, 11a, 593–601.
Maragos, C. M. (2004). Emerging technologies for mycotoxin detection. Journal of Toxicology, 23(2&3), 317–344.
Rasch, C., Kumke, M., & Löhmannsröben, H.-G. (2008). Mykotoxine in getreide spektroskopisch erfassen. Nachrichten aus der Chemie, 11, 1154–1158.
Rasch, C., Kumke, M., Löhmannsröben, H.-G. (2009) Optical sensing of fungi and mycotoxins on grains. In: Proceedings of the ANAKON, 17–20 March 2009 (p. 80). Berlin, Germany.
Rasch, C., Böttcher, M., & Kumke, M. (2010). Determination of aflatoxin B1 in alcoholic beverages: Comparison of one- and two-photon-induced fluorescence. Analytical and Bioanalytical Chemistry, 397(1), 87–92.
Rumi, M., et al. (2000). Structure-property relationships for two-photon absorbing chromophores: Bis-donor diphenylpolyene and bis(styryl)benzene derivatives. Journal of the American Chemical Society, 122, 9500–9510.
Scott, P. M. (1996). Mycotoxins transmitted into beer from contaminated grains during brewing. Journal of AOAC International, 79, 875–882.
Singh, C. B., et al. (2010). Wavelet analysis of signals in agriculture and food quality inspection. Food and Bioprocess Technology, 3(1), 2–12.
Shenderey, C., et al. (2010). NIRS detection of moldy core in apples. Food and Bioprocess Technology, 3(1), 79–86.
Steinbrück, D., Rasch, C., & Kumke, M. (2008). Photophysics of ochratoxin A in aqueous solution. Zeitschrift für Naturforschung, 63b, 1321–1326.
Sydenham, E. W., Thiel, P. G., & Vleggaar, R. (1996). Physicochemical data for some selected fusarium toxins. Journal of AOAC International, 79(6), 1365–1379.
Weidenbörner, M. (2008). Mycotoxins in foodstuffs. Berlin: Springer.
Williams, P. (2006a). Near-infrared spectroscopy of cereals. In J. M. Griffiths & V. R. Chalmers (Eds.), Handbook of vibrational spectroscopy. New York: Wiley.
Williams, P. (2006b). Near-infrared spectroscopy in food science and technology. Hoboken: Wiley.