Wound Macrophages Express TGF-α and Other Growth Factors in Vivo: Analysis by mRNA Phenotyping

American Association for the Advancement of Science (AAAS) - Tập 241 Số 4866 - Trang 708-712 - 1988
Daniel A. Rappolee1, Mark David2, Michael J. Banda1, Zena Werb1
1Laboratory of Radiobiology and Environmental Health, University of California, San Francisco, CA 94143.
2Department of Molecular Biology, Cetus Corporation, Emeryville, CA 94608.

Tóm tắt

The presence of macrophages is required for the regeneration of many cell types during wound healing. Macrophages have been reported to express a wide range of mitogenic factors and cytokines, but none of these factors has been shown in vivo to sustain all the wound-healing processes. It has been suggested that transforming growth factor-α (TGF-α) may mediate angiogenesis, epidermal regrowth, and formation of granulation tissue in vivo. Macrophages isolated from a wound site, and not exposed to cell culture conditions, expressed messenger RNA transcripts for TGF-α, TGF-β, platelet-derived growth factor A-chain, and insulin-like growth factor-1. The expression of these transcripts was determined by a novel method for RNA analysis in which low numbers of mouse macrophages were isolated from wound cylinders, their RNA was purified and reverse-transcribed, and the complementary DNA was amplified in a polymerase chain reaction primed with growth factor sequence-specific primers. This single-cell RNA phenotyping procedure is rapid and has the potential for quantification, and mRNA transcripts from a single cell or a few cells can be unambiguously demonstrated, with the simultaneous analysis of several mRNA species. Macrophages from wounds expressed TGF-α antigen, and wound fluids contained TGF-α. Elicited macrophages in culture also expressed TGF-α transcripts and polypeptide in a time-dependent manner after stimulation with modified low-density lipoproteins and lipopolysaccharide endotoxin, which are characteristic of the activators found in injured tissues.

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American Association for the Advancement of Science (AAAS)

Tập 241 Số 4866

708-712

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