Current-generation high-throughput sequencing: deepening insights into mammalian transcriptomes

Genes and Development - Tập 23 Số 12 - Trang 1379-1386 - 2009
Benjamin J. Blencowe1,2, Sidrah Ahmad1,2, Leo J. Lee1,3
1Banting and Best Department of Medical Research, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada
2Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada
3Department of Electrical and Computer Engineering, University of Toronto, Toronto, Ontario M5S 3G4, Canada

Tóm tắt

Recent papers have described the first application of high-throughput sequencing (HTS) technologies to the characterization of transcriptomes. These studies emphasize the tremendous power of this new technology, in terms of both profiling coverage and quantitative accuracy. Initial discoveries include the detection of substantial new transcript complexity, the elucidation of binding maps and regulatory properties of RNA-binding proteins, and new insights into the links between different steps in pre-mRNA processing. We review these findings, focusing on results from profiling mammalian transcriptomes. The strengths and limitations of HTS relative to microarray profiling are discussed. We also consider how future advances in HTS technology are likely to transform our understanding of integrated cellular networks operating at the RNA level.

Từ khóa


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Genes and Development

Tập 23 Số 12

1379-1386

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