Effects of human parathyroid hormone 1–34 on bone loss and lumbar intervertebral disc degeneration in ovariectomized rats

International Orthopaedics - Tập 42 - Trang 1183-1190 - 2018
Yang Luo1,2, Shu-yang Li3, Fa-ming Tian4, Hui-ping Song1, Ying-ze Zhang2, Liu Zhang1
1Department of Orthopedic Surgery, Affiliated Hospital of North China University of Science and Technology, Tangshan, People’s Republic of China
2Department of Orthopedic Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, People’s Republic of China
3College of Traditional Chinese Medicine, Inner Mongolia Medical University, Huhehaote, People’s Republic of China
4Medical Research Center, North China University of Science and Technology, Tangshan, People’s Republic of China

Tóm tắt

Lumbar intervertebral disc degeneration is a common cause of lower back pain that affects the physical and mental health of patients and increases social burden. Parathyroid hormone has been reported to be effective at inhibiting disc degeneration; however, these effects have not been fully established in vivo in ovariectomized (OVX) rats. Thus, in this study, we aimed to address this issue and examine the effects of parathyroid hormone treatment in OVX rats. Thirty female Sprague-Dawley rats, three months-old, were subjected to sham or ovariectomy surgery. Twelve weeks postsurgery, OVX rats were treated with either human parathyroid hormone [hPTH(1–34), 30 μg/kg/day] or vehicle (normal saline (NS)) treatment. The L3–6 spinal segments were harvested after 12 weeks treatment. Bone mineral density (BMD), micro-architectural parameters, and biomechanical assessment were measured at the lumbar vertebral bodies. Histology and immunohistochemistry were performed to analyze the characteristics of the lumbar intervertebral discs. OVX + PTH rats had significantly higher BMD, percentage bone volume density, trabecular thickness, and biomechanical strength compared with those in Sham and OVX + NS rats. Histology and immunostaining revealed that disc degeneration was not significantly different between the OVX + NS rats and the OVX + PTH rats, compared with the Sham group; the structure of nucleus pulposus was disordered, the expression of collagen I was increased, and collagen II and aggrecan were decreased. These findings confirmed that hPTH(1–34) treatment has substantial anabolic effects on bone mass and trabecular micro-architecture, while the excessively enhanced bone mass and strength were coupled with a non-significant effect on the disc degeneration in ovariectomized rats.

Tài liệu tham khảo

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