Detection of Heat-Labile Escherichia coli Enterotoxin with the Use of Adrenal Cells in Tissue Culture

American Association for the Advancement of Science (AAAS) - Tập 183 Số 4122 - Trang 334-336 - 1974
Sam T. Donta1, Harley W. Moon2, S. C. Whipp2
1Department of Medicine, University of Iowa Hospital and Veterans Administration Hospital, Iowa City 52240
2National Animal Disease Laboratory, Ames, Iowa 50010

Tóm tắt

Cell-free culture filtrates of heat-labile enterotoxin-producing strains of Escherichia coli are capable of inducing morphological changes and steroidogenesis in monolayer cultures of adrenal cells. These tissue culture changes are similar to those induced by cholera enterotoxin and cannot be effected by culture filtrates of other enterotoxigenic or enteropathogenic types of bacteria. The results of the tissue culture studies correlated well with those done in the standard intestinal-loop systems and suggest that this tissue culture system could be used to significantly aid epidemiological and molecular studies with heat-labile Escherichia coli enterotoxin.

Từ khóa


Tài liệu tham khảo

DONTA, S. T., NATURE-NEW BIOLOGY 243: 246 (1973).

Donta S. T. in preparation.

EVANS, D.G., DIFFERENCES IN RESPONSE OF RABBIT SMALL-INTESTINE TO HEAT-LABILE AND HEAT-STABLE ENTEROTOXINS OF ESCHERICHIA-COLI, INFECTION AND IMMUNITY 7: 873 (1973).

EVANS, D. J., NATURE-NEW BIOLOGY 236: 137 (1972).

GYLES, G.L., ANN NY ACAD SCI 176: 314 (1971).

KIMBERG, D.V., STIMULATION OF INTESTINAL MUCOSAL ADENYL CYCLASE BY CHOLERA ENTEROTOXIN AND PROSTAGLANDINS, JOURNAL OF CLINICAL INVESTIGATION 50: 1218 (1971).

MASHITER, K, EFFECTS OF CHOLERA AND ESCHERICHIA-COLI ENTEROTOXINS ON CYCLIC ADENOSINE 3',5'-MONOPHOSPHATE LEVELS AND INTERMEDIARY METABOLISM IN THYROID, ENDOCRINOLOGY 92: 541 (1973).

MOON, H.W., RESPONSE OF RABBIT ILEAL LOOP TO CELL-FREE PRODUCTS FROM ESCHERICHIA-COLI ENTEROPATHOGENIC FOR SWINE, JOURNAL OF INFECTIOUS DISEASES 121: 182 (1970).

PIERCE, N.F., VIBRIO-CHOLERAE ENTEROTOXIN AND ITS MODE OF ACTION, BACTERIOLOGICAL REVIEWS 35: 1 (1971).

SHARP, GWG, STIMULATION OF INTESTINAL ADENYL CYCLASE BY CHOLERA TOXIN, NATURE 229: 266 (1971).

SMITH, H. W., JOURNAL OF GENERAL MICROBIOLOGY 52: 319 (1968).

Thông tin
Thông tin xuất bản

American Association for the Advancement of Science (AAAS)

Tập 183 Số 4122

334-336

Thông tin tác giả