Neuroprotective effect of anthocyanins on acetylcholinesterase activity and attenuation of scopolamine‐induced amnesia in rats

International Journal of Developmental Neuroscience - Tập 33 - Trang 88-97 - 2014
Jessié M. Gutierres1, Fabiano B. Carvalho2, Maria Rosa C. Schetinger1, Paula Agostinho3, Patricia C. Marisco1, Juliano M. Vieira1, Michele M. Rosa1, Crystiani Bohnert1, Maribel A. Rubin1, Vera M. Morsch1, Roselia Spanevello4, Cinthia M. Mazzanti2
1Departamento de Química, Centro de Ciências Naturais e Exatas Universidade Federal de Santa Maria, Santa Maria, RS 97105-900, Brazil
2Setor de Bioquímica e Biologia Molecular do Laboratório de Terapia Celular, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria RS 97105-900, Brazil
3Center for Neuroscience and Cell Biology, Faculty of Medicine, Biochemistry Institute, University of Coimbra, 3004 Coimbra, Portugal
4Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário, Capão do Leão, Pelotas RS 96010-900, Brazil

Tóm tắt

AbstractAnthocyanins are a group of natural phenolic compounds responsible for the color to plants and fruits. These compounds might have beneficial effects on memory and have antioxidant properties. In the present study we have investigated the therapeutic efficacy of anthocyanins in an animal model of cognitive deficits, associated to Alzheimer's disease, induced by scopolamine. We evaluated whether anthocyanins protect the effects caused by SCO on nitrite/nitrate (NOx) levels and Na+,K+‐ATPase and Ca2+‐ATPase and acetylcholinesterase (AChE) activities in the cerebral cortex and hippocampus (of rats. We used 4 different groups of animals: control (CTRL), anthocyanins treated (ANT), scopolamine‐challenged (SCO), and scopolamine + anthocyanins (SCO + ANT). After seven days of treatment with ANT (200 mg kg−1; oral), the animals were SCO injected (1 mg kg−1; IP) and were performed the behavior tests, and submitted to euthanasia. A memory deficit was found in SCO group, but ANT treatment prevented this impairment of memory (P < 0.05). The ANT treatment per se had an anxiolytic effect. AChE activity was increased in both in cortex and hippocampus of SCO group, this effect was significantly attenuated by ANT (P < 0.05). SCO decreased Na+,K+‐ATPase and Ca2+‐ATPase activities in hippocampus, and ANT was able to significantly (P < 0.05) prevent these effects. No significant alteration was found on NOx levels among the groups. In conclusion, the ANT is able to regulate cholinergic neurotransmission and restore the Na+,K+‐ATPase and Ca2+‐ATPase activities, and also prevented memory deficits caused by scopolamine administration.

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International Journal of Developmental Neuroscience

Tập 33

88-97

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