Molecular mechanism of violacein-mediated human leukemia cell death

Blood - Tập 104 - Trang 1459-1464 - 2004
Carmen Veríssima Ferreira, Carina L. Bos, Henri H. Versteeg, Giselle Z. Justo, Nelson Durán, Maikel P. Peppelenbosch

Tóm tắt

Abstract Violacein, a pigment isolated from Chromobacterium violaceum in the Amazon River, presents diverse biologic properties and attracts interest as a consequence of its antileukemic activity. Elucidation of the molecular mechanism mediating this activity will provide further relevant information for understanding its effects on the cellular physiology of untransformed cells and for considering its possible clinical application. Here, we show that violacein causes apoptosis in HL60 leukemic cells but is ineffective in this respect in other types of leukemia cells or in normal human lymphocytes and monocytes. Violacein cytotoxicity in HL60 cells was preceded by activation of caspase 8, transcription of nuclear factor κB (NF-κB) target genes, and p38 mitogen-activated protein (MAP) kinase activation. Thus, violacein effects resemble tumor necrosis factor α (TNF-α) signal transduction in these cells. Accordingly, infliximab, an antibody that antagonizes TNF-α–induced signaling abolished the biologic activity of violacein. Moreover, violacein directly activated TNF receptor 1 signaling, because a violacein-dependent association of TNF receptor-associated factor 2 (TRAF2) to this TNF receptor was observed in coimmunoprecipitation experiments. Hence, violacein represents the first member of a novel class of cytotoxic drugs mediating apoptosis of HL60 cells by way of the specific activation of TNF receptor 1.

Tài liệu tham khảo

Durán N, Menck CFM. Chromobacterium violaceum: a review of pharmacological and industrial perspectives. Crit Rev Microbiol. 2001;27: 201-222. Rettori D, Durán N. Production, extraction and purification of violacein: an antibiotic produced by Chromobacterium violaceum. World J Microbiol Biotechnol. 1998;14: 685-688. Bromberg N, Durán N. Violacein transformation by peroxidases and oxidases: implications on its biological properties. J Mol Catalysis B Enzymatic. 2001;11: 463-467. Leon LL, Miranda CC, De Souza AO, DuránN. Antileishmanial activity of the violacein extracted from Chromobacterium violaceum. J Antimicrob Chemother. 2001;48: 449-450. Durán N, Justo GZ, Melo PS, et al. Evaluation of the antiulcerogenic activity of violacein and its modulation by the inclusion complexation with β-cyclodextrin. Can J Physiol Pharmacol. 2003;81: 387-396. Melo PS, Justo GZ, De Azevedo MBM, DuránN, Haun M. Violacein and its beta-cyclodextrin complexes induce apoptosis and differentiation in HL60 cells. Toxicology. 2003;186: 217-225. Uzunoglu S, Uslu R, Tobu M, et al. Augmentation of methylprednisolone-induced differentiation of myeloid leukemia cells by serine threonine protein phosphatase inhibitors. Leuk Res. 1999;23: 507-512. Wang XN, Studzinski GP. Inhibition of p38MAP kinase potentiates the JNK/SAPK pathway and AP-1 activity in monocytic but not in macrophage or granulocytic differentiation of HL60 cells. J Cell Biochem. 2001;82: 68-77. Sundstrom C, Nilsson K. Establishment and characterization of a human histiocytic lymphoma cell line (U937). Int J Cancer. 1976;17: 565-577. Olsson I, Gullberg U, Ivhed I, Nilsson K. Induction of differentiation of the human histiocytic lymphoma cell line U-937 by 1 alpha,25-dihydroxycholecalciferol. Cancer Res. 1983;43: 5862-5867. Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood. 1975;45: 321-334. Andersson LC, Nilsson K, Gahmberg CG. K562: a human erythroleukemic cell line. Int J Cancer. 1979;23: 143-147. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Meth. 1983;65: 55-63. Hartree EF. Determination of proteins: a modification of Lowry method that give a linear photometric response. Anal Biochem. 1972;48: 422-427. Van Den Brande JMH, Braat H, Van Den Brink GR, et al. Infliximab but not etanercept induces apoptosis in lamina propria T-lymphocytes from patients with Crohn's disease. Gastroenterology. 2003;124: 1774-1785. O'Gorman DM, Cotter TG. Molecular signals in anti-apoptotic survival pathways. Leukemia. 2001;15: 21-34. Makin G, Dive C. Apoptosis and cancer chemotherapy. Trends Cell Biol Sci. 2001;11: S22-S26. Martelli AM, Tazzari PL, Tabellini G, et al. A new selective AKT pharmacological inhibitor reduces resistance to chemotherapeutic drugs, TRAIL, all-trans-retinoic acid, and ionizing radiation of human leukemia cells. Leukemia. 2003;17: 1794-1805. Freire AG, Melo PS, Haun M, Dúran N, Aoyama H, Ferreira CV. Cytotoxic effect of the diterpene lactone dehydrocrotonin from Croton cajucara on human promyelocytic leukemia cells. Planta Medica. 2003;69: 67-69. Deng Y, Ren X, Yang L, Lin Y, Wu X. A JNK dependent pathway is required for TNFα-induced apoptosis. Cell. 2003;115: 61-70. Bowie A, O'Neill LA. Oxidative stress and nuclear factor-kappa B activation: a reassessment of the evidence in the light of recent discoveries. Biochem Pharmacol. 2000;59: 13-23. Karin M, Lin A. NF-kappa B at the crossroads of life and death. Nature Immunol. 2002;3: 221-227. Wen L, Zhuang L, Luo X, Wei P. TL1A-induced NF-kappa B activation and c-IAP2 production prevent DR3-mediated apoptosis in TF-1 cells. J Biol Chem. 2003;278: 39251-39258. Steinman RA, Huang JP, Yaroslavskiy B, Goff JP, Ball ED, Nguyen A. Regulation of p21(WAF1) expression during normal myeloid differentiation. Blood. 1998;91: 4531-4542. Zada AAP, Singh SM, Reddy VA, et al. Down-regulation of c-Jun expression and cell cycle regulatory molecules in acute myeloid leukemia cells upon CD44 ligation. Oncogene. 2003;22: 2296-2308. Lavelle D, DeSimone J, Hankewych M, Kousnetzova T, Chen YH. Decitabine induces cell cycle arrest at the G1 phase via p21(WAF1) and the G2/M phase via the p38 MAP kinase pathway. Leuk Res. 2003;27: 999-1007. Wang YJ, Wu TR, Cai SY, Welte T, Chin YE. Stat1 as a component of tumor necrosis factor alpha receptor 1-TRADD signaling complex to inhibit NF-Kappa B activation. Mol Cell Biol. 2000;20: 4505-4512.
Thông tin
Thông tin xuất bản

Blood

Tập 104

1459-1464

Thông tin tác giả