Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro
Tóm tắt
The objective of the current study is to determine the effect of light quality on enhancement of growth, phytochemicals, antioxidant potential, and antioxidant enzyme activities at in vitro cultures of Rehmannia glutinosa Libosch. In vitro-grown shoot tip explants were cultured on the plant growth regulator (PGR)-free Murashige and Skoog (MS) medium and cultured under a conventional cool white fluorescent light (control), blue light emitting diode (LED) light or red LED light. After four weeks, the growth traits along with total phenol content, total flavonoid content, free radical scavenging activities, and antioxidant enzyme activities were measured. Interestingly, the blue or red LED treatments showed a significant increase in growth parameters compared with the cool white florescent light. In addition, the LED treatments increased the total phenol and flavonoid levels in leaf and root extracts. Furthermore, data on the total antioxidant capacity, reducing power potential, and DPPH radical scavenging capacity also revealed the enhancement of antioxidant capacity under both blue and red LED treatments. Especially, the blue LED treatment significantly increased the antioxidant enzyme activities in both the leaf and root, followed by the red LED treatment. Modulation in the spectral quality particularly by the blue LED induced the antioxidant defense line and was directly correlated with the enhancement of phytochemicals. Therefore, the incorporation of blue or red LED light sources during in vitro propagation of R. glutinosa can be a beneficial way to increase the medicinal values of the plant.
Tài liệu tham khảo
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