Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro

Reproduction, Fertility and Development - Tập 20 Số 5 - Trang 640 - 2008
Roberta Nogueira Chaves1, F.S. Martins1, M. V. A. Saraiva1, J.J.H. Celestino1, C.A.P. Lopes1, João Carlos Correia1, Isabel Bezerra Lima Verde1, Maria Helena Tavares de Matos1, Sônia Nair Báo2, Khesller Patrícia Olázia Name2, C.C. Campello1, J.R.V. Silva3, J.R. Figueiredo1
1Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Av. Paranjana 1700, Campus Itapery, Fortaleza 60740-903, CE, Brazil.
2Laboratory of Electron Microscopy, Department of Cell Biology, University of Brasilia, Av. Asa Norte, Campus Darcy Ribeiro, Brasilia 70910-900, DF, Brazil.
3Biotechnology Nucleus of Sobral (NUBIS)–State University of Vale of Acaraú, Gerardo Rangel s/n, Campus Derby, Sobral 62040-370, CE, Brazil.

Tóm tắt

The aim of the present study was to evaluate the effects of storage of goat ovarian fragments at different temperatures and for different incubation times on the viability and growth of cultured preantral follicles in vitro. Caprine ovaries were collected and divided into 19 fragments, with one fragment being fixed immediately (fresh control). The remaining fragments were placed in minimal essential medium (MEM) and maintained at 4, 20 or 35°C for 2 or 4 h. After each incubation period, some of the fragments were fixed (non-cultured), whereas others were cultured in vitro for 1 or 7 days. Fragments were processed to enable routine histological and transmission electron microscopic examination. After 7 days of culture, only ovarian fragments stored at 4°C for 4 h maintained a percentage of morphologically normal follicles similar to that in the fresh control. For all other treatments groups, there was a significant increase in follicular activation observed. In addition, there was an increase in oocyte and follicular diameter after culture of ovarian cortex that had been chilled previously at 4°C for 2 or 4 h. In conclusion, the present study demonstrated that chilling ovarian fragments at 4°C during transportation is best for maintaining follicle viability and to increase follicular growth during in vitro culture.

Từ khóa


Tài liệu tham khảo

Andrade, 2002, Small Rumin. Res., 43, 235, 10.1016/S0921-4488(02)00018-4

Braw-Tal, 1997, J. Reprod. Fertil., 109, 165, 10.1530/jrf.0.1090165

Carvalho, 2001, Anim. Reprod. Sci., 66, 195, 10.1016/S0378-4320(01)00085-9

Cecconi, 1999, Biol. Reprod., 60, 594, 10.1095/biolreprod60.3.594

Clark, 2004, Biol. Reprod., 71, 1071, 10.1095/biolreprod.104.029652

Costa, 2002, Braz. J. Vet. Res. Anim. Sci., 39, 324, 10.1590/S1413-95962002000600009

Figueiredo, 1994, Theriogenology, 41, 1333, 10.1016/0093-691X(94)90492-2

Figueiredo, 2007, Rev. Bras. Reprod. Anim., 31, 143

Gao, 2007, J. Reprod. Contracept., 18, 25, 10.1016/S1001-7844(07)60004-2

Jewgenow, 1997, Mol. Reprod. Dev., 48, 350, 10.1002/(SICI)1098-2795(199711)48:3<350::AID-MRD8>3.0.CO;2-Q

Lucci, 2004, Theriogenology, 61, 461, 10.1016/S0093-691X(03)00226-7

Lucci, 2007, Reprod. Domest. Anim., 42, 76, 10.1111/j.1439-0531.2006.00734.x

Martins, 2005, Anim. Reprod., 2, 106

Matos, 2004, Theriogenology, 62, 65, 10.1016/j.theriogenology.2003.07.025

Matos, 2007, Zygote, 15, 173, 10.1017/S0967199407004169

McGee, 2000, Endocr. Rev., 21, 200

Miyano, 2007, Soc. Reprod. Fertil. Suppl., 63, 531

Pedersen, 2004, Theriogenology, 62, 468, 10.1016/j.theriogenology.2003.10.006

Saha, 2000, Anim. Reprod. Sci., 63, 27, 10.1016/S0378-4320(00)00162-7

Salehi, 2004, Cryobiology, 48, 245, 10.1016/j.cryobiol.2004.01.008

Santos, 2002, Braz. J. Vet. Res. Anim. Sci., 39, 254

Silva, 2000, Theriogenology, 54, 809, 10.1016/S0093-691X(00)00392-7

Silva, 2004, Theriogenology, 61, 1691, 10.1016/j.theriogenology.2003.09.014

Tas, 2006, Anim. Reprod. Sci., 96, 30, 10.1016/j.anireprosci.2005.11.001

Telfer, 2000, Mol. Cell. Endocrinol., 163, 117, 10.1016/S0303-7207(00)00216-1

Wongsrikeao, 2005, J. Reprod. Dev., 51, 87, 10.1262/jrd.51.87

Thông tin
Thông tin xuất bản

Reproduction, Fertility and Development

Tập 20 Số 5

640

Thông tin tác giả