RNA-Guided Human Genome Engineering via Cas9

American Association for the Advancement of Science (AAAS) - Tập 339 Số 6121 - Trang 823-826 - 2013
Prashant Mali1, Luhan Yang2,1, Kevin M. Esvelt3, John Aach1, Marc Güell1, James J. DiCarlo4, Julie E. Norville1, George M. Church1,3
1Department of Genetics, Harvard Medical School, Boston, MA 02115 USA
2Biological and Biomedical Sciences Program, Harvard Medical School, Boston, MA 02115, USA
3Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA 02138 USA
4Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA

Tóm tắt

Genome Editing Clustered regularly interspaced short palindromic repeats (CRISPR) function as part of an adaptive immune system in a range of prokaryotes: Invading phage and plasmid DNA is targeted for cleavage by complementary CRISPR RNAs (crRNAs) bound to a CRISPR-associated endonuclease (see the Perspective by van der Oost ). Cong et al. (p. 819 , published online 3 January) and Mali et al. (p. 823 , published online 3 January) adapted this defense system to function as a genome editing tool in eukaryotic cells.

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American Association for the Advancement of Science (AAAS)

Tập 339 Số 6121

823-826

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