ZntR is a Zn(II)‐responsive MerR‐like transcriptional regulator of <i>zntA</i> in <i>Escherichia coli</i>

Molecular Microbiology - Tập 31 Số 3 - Trang 893-902 - 1999
Kathryn R. Brocklehurst1, Jon L. Hobman2, Blair Lawley1, Lindsay Blank2, Samantha J. Marshall1, Nigel L. Brown2, Andrew P. Morby1
1School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF1 3US, UK.,
2School of Biological Sciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

Tóm tắt

We have identified the promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance (mer ) operons, including a long spacer region containing an inverted repeat sequence. The gene encoding the transcriptional regulator of PzntA, designated zntR, has been identified from genome sequence data, by expression of the gene product and by insertional inactivation/complementation. The ZntR product is a member of the MerR family of transcriptional regulators and appears to act as a hypersensitive transcriptional switch. A hybrid MerR/ZntR protein has been constructed and indicates that the C‐terminal region of ZntR recognizes Zn(II).

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