Specific Association of Human Telomerase Activity with Immortal Cells and Cancer

American Association for the Advancement of Science (AAAS) - Tập 266 Số 5193 - Trang 2011-2015 - 1994
Nam W. Kim1, A. Kleinhofs2, Karen R. Prowse1, Calvin B. Harley1, Michael D. West1, Peter L. C. Ho1, Gina M. Coviello1, Woodring E. Wright2, Scott L. Weinrich1, Jerry W. Shay2
1Geron Corporation, Menlo Park, CA 94025.
2University of Texas Southwestern Medical Center at Dallas, Department of Cell Biology and Neurosciences, TX 75235-9039, USA.

Tóm tắt

Synthesis of DNA at chromosome ends by telomerase may be necessary for indefinite proliferation of human cells. A highly sensitive assay for measuring telomerase activity was developed. In cultured cells representing 18 different human tissues, 98 of 100 immortal and none of 22 mortal populations were positive for telomerase. Similarly, 90 of 101 biopsies representing 12 human tumor types and none of 50 normal somatic tissues were positive. Normal ovaries and testes were positive, but benign tumors such as fibroids were negative. Thus, telomerase appears to be stringently repressed in normal human somatic tissues but reactivated in cancer, where immortal cells are likely required to maintain tumor growth.

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Tài liệu tham khảo

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American Association for the Advancement of Science (AAAS)

Tập 266 Số 5193

2011-2015

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