Depth-dependent responses to solar ultraviolet radiation and oxidative stress in the zooxanthellate coral Acropora microphthalma
Tóm tắt
Colonies of Acropora microphthalma (Verrill 1869) were transferred from depths of 2 to 3, 10, 20, and 30 m to UV-transparent and UV-opaque respirometry chambers placed at 1 m depth at Bowl Reef, Great Barrier Reef, in March 1989. Peak rates of photosynthesis in colonies originating at 2 and 10 m were unaffected by solar ultraviolet (UV) radiation at 1 m, whereas photosynthesis showed 30 and 38% inhibition in colonies transferred from 20 and 30 m, respectively. This differential sensitivity of corals to UV radiation was consistent with the five- to tenfold higher concentrations of UV-absorbing, mycosporine-like amino acids (MAAs, putative defenses against UV) in 2- and 10-m colonies compared with 20- and 30-m colonies. Photosynthesis in zooxanthellae freshly isolated from 2- and 10-m corals, however, was inhibited by UV, indicating that the host's tissues, which contain 95% of the total MAAs in corals at these depths, are the first line of defense against solar UV and provide protection to their endosymbiotic algae. The general bathymetric decline in the activities of the antioxidant enzyme superoxide dismutase (SOD) in the host, and SOD, catalase, and ascorbate peroxidase in the zooxanthellae, is related to the decrease in potential for photooxidative stress with increasing depth.
Tài liệu tham khảo
Asada K, Takahashi, M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CB, Arntzen CJ (eds) Photoinhibition. Elsevier Science Publishers, Amsterdam, pp 227–287
Baker K, Smith RC, Green AES (1980) Middle ultraviolet reaching the ocean surface. Photochem Photobiol 32:367–374
Battey JF, Porter JW (1988) Photoadaptation as a whole organism response in Montastrea annularis. Proc 6th int coral Reef Symp, 6:79–87 [Choat JH et al (eds) Sixth International Coral Reef Symposium Executive Committee, Townsville]
Blumthaler M, Ambach W (1990) Indication of increasing solar ultraviolet-B radiation flux in alpine regions. Science, NY 248: 206–208
Bradford, MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein using the principle of proteindye binding. Analyt Biochem 72:248–254
Cadenas E (1989) Biochemistry of oxygen toxicity. A Rev Biochem 58:79–110
Caldwell MM (1971) Solar ultraviolet radiation and the growth and development of higher plants. In: Giese AC (ed) Photophysiology, Vol. 6. Academic Press, New York, pp 131–177
Carroll AK, Shick JM (1993) Dietary accumulation of UV-absorbing compounds in the ovaries of the green sea urchin. Am Zool 33:62A (abstract)
Cavanaugh GM (1975) Formulae and methods VI of the Marine Biological Laboratory Chemical Room. Marine Biological Laboratory, Woods Hole, Massachusetts
Chalker BE, Carr K, Gill E (1985) Measurement of primary production and calcification in situ on coral reefs using electrode techniques. Proc 5th int coral Reef Congr 2:167–172 [Gabrié C et al (eds) Antenne Museum-EPHE, Moorea, French Polynesia]
Chalker BE, Dunlap WC (1983) Primary production and photoadaptation by corals on the Great Barrier Reef. In: Baker JT, Carter RM, Sammarco PW, Stark KP (eds) Proceedings of the Inaugural Great Barrier Reef Conference. James Cook University Press, Townsville, Australia, pp 293–298
Cullen JJ, Lesser MP (1991) Inhibition of photosynthesis by ultraviolet radiation as a function of dose and dosage rate: results for a marine diatom. Mar Biol 111:183–190
Cullen JJ, Neale PJ, Lesser MP (1992) Biological weighting function for the inhibition of phytoplankton photosynthesis by ultraviolet radiation. Science, NY 258:646–650
Drollet JH, Glaziou P, Martin PMV (1993) A study of mucus from the solitary coral Fungia fungites (Scleractinia: Fungiidae) in relation to photobiological UV adaptation. Mar Biol 115:263–266
Dubinsky Z, Falkowski PG, Post AF, van Hes UM (1987) A system for measuring phytoplankton photosynthesis in a defined light field with an oxygen electrode. J Plankton Res 9:607–612
Dunlap WC, Chalker BE (1986) Identification and quantitation of near-UV absorbing compounds (S-320) in a hermatypic scleractinian. Coral Reefs 5:155–159
Dunlap WC, Chalker BE, Oliver JK (1986) Bathymetric adaptations of reef-building corals at Davies Reef, Great Barrier Reef, Australia. III. UV-B absorbing compounds. J exp mar Biol Ecol 104:239–248
Dunlap WC, Williams DM, Chalker BE, Banaszak AT (1989) Biochemical photoadaptation in vision: U.V.-absorbing pigments in fish eye tissues. Comp Biochem Physiol 93B:601–607
Dykens JA, Shick JM (1982) Oxygen production by endosymbiotic algae controls superoxide dismutase activity in their animal host. Nature, Lond 297:579–580
Dykens JA, Shick JM (1984) Photobiology of the symbiotic sea anemone Anthopleura elegantissima: Defenses against photodynamic effects, and seasonal photoacclimatization. Biol Bull mar Biol Lab, Woods Hole 167:683–697
Dykens JA, Shick JM, Benoit C, Buettner GR, Winston GW (1992) Oxygen radical production in the sea anemone Anthopleura elegantissima and its endosymbiotic algae. J exp Biol 168:219–241
Edmunds PJ (1988) Post-illumination stimulation of respiration of respiration rate in the coral Porites porites. Coral Reefs 7:7–9
Falkowski PG, Jokiel PL, Kinzie RA III (1990) Irradiance and corals. In: Dubinsky Z (ed) Coral reefs. Ecosystems of the world, Vol. 25. Elsevier, Amsterdam, pp 89–107
Fleischmann EM (1989) The measurement and penetration of ultraviolet radiation into tropical marine water. Limnol Oceanogr 34:1623–1629
Frederick JE, Snell HE (1988) Ultraviolet radiation levels during the Antarctic Spring. Science, NY 241:438–440
Gattuso J-P, Yellowlees D, Lesser MP (1993) Depth- and light-dependent variation of carbon partitioning and utilization in the zooxanthellate scleractinian coral Stylophora pistillata. Mar Ecol Prog Ser 92:267–276
Gleason DF (1993) Differential effects of ultraviolet radiation on green and brown morphs of the Caribbean coral Porites astreoides. Limnol Oceanogr 38:1452–1463
Gleason DF, Wellington GM (1993) Ultraviolet radiation and coral bleaching. Nature, Lond 365:836–838
Glynn PW, Imai R, Sakai K, Nakano Y, Yamazato K (1994) Experimental responses of Okinawan (Ryukyu Islands, Japan) reef corals to high sea temperature and UV radiation. Proc 7th int coral Reef Symp (1992) 1:27–37 [Richmond J (ed) Seventh International Coral Reef Symposium Committee, University of Guam]
Hirata Y, Uemura D, Ueda K, Takano S (1979) Several compounds from Palythoa tuberculosa (Coelenterata). Pure appl Chem 51:1875–1883
Jerlov NG (1950) Ultra-violet radiation in the sea. Nature, Lond 116:111–112
Jerlov NG (1968) Optical oceanography. Elsevier, Amsterdam
Jokiel PL (1980) Solar ultraviolet radiation and coral reef epifauna. Science, NY 207:1069–1071
Jokiel PL, York RH, Jr (1982) Solar ultraviolet photobiology of the reef coral Pocillopora damicornis and symbiotic zooxanthellae. Bull mar Sci 32:301–315
Jones LW, Kok B (1966) Photoinhibition of chloroplast reactions. I. Kinetics and action spectra. Pl Physiol 41:1037–1043
Kerr JB, McElroy CT (1993) Evidence for large upward trends of ultraviolet-B radiation linked to ozone depletion. Science, NY 262:1032–1034
Kinzie RA, III (1993) Effects of ambient levels of solar ultraviolet radiation on zooxanthellae and photosynthesis of the reef coral Montipora verrucosa. Mar Biol 116:319–327
Ku H-K, Brunk UT, Sohal RS (1993) Relationship between mitochondrial superoxide and hydrogen peroxide production and longevity of mammalian species. Free Radical Biol Med 15:621–627
Lesser MP (1989) Photobiology of natural populations of zooxanthellae from the sea anemone Aiptasia pallida: assessment of the host's role in protection against ultraviolet radiation. Cytometry 10:653–658
Lesser MP, Shick JM (1989a) Photoadaptation and defenses against oxygen toxicity in zooxanthellae from natural populations of symbiotic cnidarians. J exp mar Biol Ecol 134:129–141
Lesser MP, Shick JM (1989b) Effects of irradiance and ultraviolet radiation on photoadaptation in the zooxanthellae of Aiptasia pallida: primary production, photoinhibition, and enzymic defenses against oxygen toxicity. Mar Biol 102:243–255
Lesser MP, Shick JM (1990) Effects of visible and ultraviolet radiation on the ultrastructure of zooxanthellae (Symbiodinium sp.) in culture and in situ. Cell Tissue Res 261:501–508
Lesser MP, Stochaj WR, Tapley DW, Shick JM (1990) Physiological mechanisms of bleaching in coral reef anthozoans: effects of irradiance, ultraviolet radiation, and temperature on the activities of protective enzymes against active oxygen. Coral Reefs 8:225–232
Masuda K, Goto M, Maruyama T, Miyachi S (1993) Adaptation of solitary corals and their zooxanthellae to low light and UV radiation. Mar Biol 117:685–691
Matta JL, Trench RK (1991) The enzymatic response of the symbiotic dinoflagellate Symbiodinium microadriaticum (Freudenthal) to growth in vitro under varied oxygen tensions. Symbiosis 11:31–45
McCloskey LR, Muscatine L (1984) Production and respiration in the Red Sea coral Stylophora pistillata as a function of depth. Proc R Soc Lond (Ser B) 222:215–230
Platt T, Gallegos CL, Harrison WG (1980) Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton. J mar Res 38:687–701
Rowan R, Powers DA (1991) A molecular genetic classification of zooxanthellae and the evolution of animal-algal symbioses. Science, NY 251:1348–1351
Scelfo GM (1985) The effects of visible and ultraviolet solar radiation on a UV-absorbing compound and chlorophyll a in a Hawaiian zoanthid. Proc 5th int coral Reef Cong 2:107–112 [Gabrié C et al (eds) Antenne Museum-EPHE, Moorea, French Polynesia]
Scelfo G (1986) Relationship between solar radiation and pigmentation of the coral Montipora verrucosa and its zooxanthellae. In: Jokiel PL, Richmond RH, Rogers RA (eds) Coral reef population biology, technical report no. 37. Hawaii Institute of Marine Biology, Honolulu, pp 440–451
Setlow RB (1974) The wavelengths in sunlight effective in producing skin cancer: a theoretical analysis. Proc natn Acad Sci USA 71:3363–3366
Shibata K (1969) Pigment and a UV-absorbing substance in corals and a blue-green alga living in the Great Barrier Reef. Pl Cell Physiol 10:325–335
Shick JM (1993) Solar UV and oxidative stress in algal-animal symbioses. In: Shima A, Ichihashi M, Fujiwara Y, Takebe H (eds) Frontiers of photobiology. Proceedings of the 11th International Congress on Photobiology. Elsevier Science Publishers, Amsterdam, pp 561–564
Shick JM, Dunlap WC, Chalker BE, Banaszak AT, Rosenzweig TK (1992) Survey of ultraviolet radiation-absorbing mycosporinelike amino acids in organs of coral reef holothuroids. Mar Ecol Prog Ser 90:139–148
Shick JM, Dunlap WC, Larsen RM (1993) Coral reef holothuroids (Echinodermata) accumulate UV-photoprotectants from their diet. Am Zool 33:62A (abstract)
Shick JM, Dykens JA (1985) Oxygen detoxification in algal-invertebrate symbioses from the Great Barrier Reef. Oecologia 66:33–41
Shick JM, Lesser MP, Stochaj WR (1991) Ultraviolet radiation and photooxidative stress in zooxanthellate Anthozoa: the sea anemone Phyllodiscus semoni and the octocoral Clavularia sp. Symbiosis 10:145–173
Siebeck O (1981) Photoreactivation and depth-dependent UV tolerance in reef coral in the Great Barrier Reef/Australia. Naturwissenschaften 68:426–428
Siebeck O (1988) Experimental investigation of UV tolerance in hermatypic corals (Scleractinia). Mar Ecol Prog Ser 43:95–103
Smith RC, Baker KS (1979) Penetration of UV-B and biologically effective dose-rates in natural waters. Photochem Photobiol 29:311–323
Smith RC, Baker KS, Holm-Hansen O, Olson RS (1980) Photoinhibition of photosynthesis in natural waters. Photochem Photobiol 31:585–592
Smith RC, Prézelin BB, Baker KS, Bidigare RR, Boucher NP, Coley T, Karentz D, MacIntyre S, Matlick HA, Menzies D, Ondrusek M, Wan Z, Waters KJ (1992) Ozone depletion: ultraviolet radiation and phytoplankton biology in Antarctic waters. Science, NY 255:952–959
Stochaj WR, Dunlap WC, Shick JM (1994) Two new UV-absorbing mycosporine-like amino acids from the sea anemone Anthopleura elegantissima and the effects of zooxanthellae and spectral irradiance on chemical composition and content. Mar Biol 118:149–156
Tapley DW, Shick JM, Smith JPS, III (1988) Defenses against oxidative stress in the symbiotic sea anemones Aiptasia pallida and Aiptasia pulchella. Am Zool 28:105A (abstract)
Tyrrell RM (1991) UVA (320–380 nm) radiation as an oxidative stress. In: Sies H (ed) Oxidative stress: oxidants and antioxidants. Academic Press, San Diego, pp 57–83
Weger HG, Herzig R, Falkowski PG, Rurpin DH (1989) Respiratory losses in the light of a marine diatom: measurements by shortterm mass spectrometry. Limnol Oceanogr 34:1153–1161