The Genetic Landscape of a Cell

American Association for the Advancement of Science (AAAS) - Tập 327 Số 5964 - Trang 425-431 - 2010
Michael Costanzo1,2, Anastasia Baryshnikova1,2, Jeremy Bellay3, Yungil Kim3, Eric D. Spear4, Carolyn S. Sevier4, Huiming Ding1,2, Judice L.Y. Koh1,2, Kiana Toufighi1,2, Sara Mostafavi1,5, Jeany Prinz1,2, Robert P. St.Onge6, Benjamin VanderSluis3, Taras Makhnevych7, Franco J. Vizeacoumar1,2, Solmaz Alizadeh1,2, Sondra Bahr1,2, Renée L. Brost1,2, Yiqun Chen1,2, Murat Cokol8, Raamesh Deshpande3, Zhijian Li1,2, Zhen-Yuan Lin9, Wendy Liang1,2, Michaela Marback1,2, Jadine Paw1,2, Bryan-Joseph San Luis1,2, Ermira Shuteriqi1,2, Amy H.Y. Tong1,2, Nydia van Dyk1,2, Iain M. Wallace1,2,10, Joseph Whitney1,5, Matthew T. Weirauch11, Guoqing Zhong1,2, Hongwei Zhu1,2, Walid Houry7, Michael Brudno1,5, Sasan Ragibizadeh12, Balázs Papp13, Csaba Pál13, Frederick P. Roth8, Guri Giaever2,10, Corey Nislow1,2, Olga G. Troyanskaya14, Howard Bussey15, Gary D. Bader1,2, Anne‐Claude Gingras9, Quaid Morris1,5,2, Philip M. Kim1,2, Chris A. Kaiser4, Chad L. Myers3, Brenda Andrews1,2, Charles Boone1,2
1Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, M5S 3E1, Canada
2Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, M5S 3E1, Canada
3Department of Computer Science and Engineering, University of Minnesota, Minneapolis, MN, 55455 USA
4Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
5Department of Computer Science, University of Toronto, Toronto, Ontario, M5S 2E4, Canada
6Department of Biochemistry, Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA.
7Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
8Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
9Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University avenue, Toronto, Ontario, M5G 1X5, Canada
10Department of Pharmacy, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
11Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, USA
12S&P Robotics, Inc., 1181 Finch Avenue West, North York, Ontario M3J 2V8, Canada.
13Institute of Biochemistry, Biological Research Center, H-6701 Szeged, Hungary.
14Department of Computer Science, Lewis-Sigler Institute for Integrative Genomics, Carl Icahn Laboratory, Princeton University, Princeton, NJ 08544, USA.
15Biology Department, McGill University, Montreal, Quebec, H3A 1B1, Canada

Tóm tắt

Making Connections

Genetic interaction profiles highlight cross-connections between bioprocesses, providing a global view of cellular pleiotropy, and enable the prediction of genetic network hubs. Costanzo et al. (p. 425 ) performed a pairwise fitness screen covering approximately one-third of all potential genetic interactions in yeast, examining 5.4 million gene-gene pairs and generating quantitative profiles for ∼75% of the genome. Of the pairwise interactions tested, about 3% of the genes investigated interact under the conditions tested. On the basis of these data, a reference map for the yeast genetic network was created.

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