champuru 1.0: a computer software for unraveling mixtures of two DNA sequences of unequal lengths

Wiley - Tập 7 Số 6 - Trang 974-977 - 2007
Jean‐François Flot1
1UMR UPMC-CNRS-MNHN-IRD 7138, Département Systématique et Évolution, Muséum National d'Histoire Naturelle, Case Postale 26, 57 rue Cuvier, 75231 Paris Cedex 05, France

Tóm tắt

Abstract

champuru is an interactive, user‐friendly web software that facilitates the deconvolution of mixed chromatograms obtained when sequencing directly mixtures of two DNA templates of unequal lengths. The program takes as input two strings of characters describing the forward and reverse chromatograms as obtained by direct sequencing and returns, most often after several iterations aimed at correcting basecalling errors, the sequences of the two templates present in the mixture. champuru was written in perl, with a web interface accessible online at http://134.157.186.185/champuru/champuru.htm.

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Tài liệu tham khảo

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